摘要
对具有药用与调味价值的植物南姜进行了组培快繁技术的研究。结果表明：南姜块茎的幼芽是理想的外植体；消毒方式选用75％乙醇30s＋0.1％HgCl2处理10-15min，污染率可降低至12％，成活率可达64％；MS＋3m／L 6-BA＋0.5mg／L NAA激素配比有利于诱导芽分化及腋芽的萌发，其诱导率可达83.3％；生根培养基采用1／2MS＋0.1mg／L NAA＋0.05mg／L 6-BA，其生根率达100％，平均生根数12-15条。
Studied tissue culture and rapid propagation of Alpinia galangal which was a medicinal and seasoning plant with promising development future. The results showed that the tender buds of Alpinia galangal＇s tubers were the optimum explants of tissue culture; 75% ethanol （30 s） and 0.1% HgCle （10-15 min. ） was the best disinfectant,and the pollution rate of explants was only 12%, with the survival rate up to 64%; MS＋3 mg/L 6-BA＋0.5 rng/L NAA was the most suitable medium for propagation in which shoots induction rate had reached to 83.3%; 1/2MS＋0.1mg/L NAA-＋0.05 mg/L 6-BA was the optimum medium for rooting in which the rooting rate reached 100%, with the average numbers of roots of 12-15.
出处
《北方园艺》
CAS
北大核心
2009年第2期18-21,共4页
Northern Horticulture
基金
国家星火计划资助项目(2005EA780080)
关键词
南姜
组织培养
快繁
Alpinia galanga
Tissue culture
Rapid propagation
