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杜氏盐藻核基质结合区结合蛋白的细胞定位 被引量：2

Cellular localization of MAR binding protein of Dunaliella salina
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摘要 为研究核基质结合区结合蛋白的功能及调控机制,PCR扩增杜氏盐藻MBP的cDNA全长序列及N端和C端序列,与绿色荧光蛋白基因融合构建真核表达载体,脂质体转染CHO细胞,Western blotting和荧光显微镜检测基因表达情况和细胞定位。结果显示:MBP及N端和C端融合蛋白成功在CHO细胞表达,MBP和C端部分定位于细胞核且聚集于核仁,N端部分分布整个细胞,说明MBP定位于细胞核且细胞定位信号位于C端,MBP可能与rRNA前体结合发挥作用。 To study the function of Matrix attachment region（MAR）binding protein（MBP）, full length, N-terminal and C-terminal of MBP gene from Dunaliella salina were amplified by RT-PCR,and fused to the green fluorescence protein gene to construct the eukaryotic vector. Then,the recombinant vectors were transfected into Chinese hamster ovary（CHO） cells by Lipofectamine 2000. Fluorescence microscope and western blotting were performed to determine the expression of the fusion proteins and cellular localization. The results showed that the fusion proteins were expressed in CHO cells, the proteins of MBP and C-terminal part were localized in the nucleus and clearly detected in nucleoli,however, the N-terminal part was distributed into all ceils.
作者 王鹏举 王天云 冯英才 刘红涛 薛乐勋
机构地区 郑州大学细胞生物学研究室 新乡医学院生物化学与分子生物学教研室
出处 《广西植物》 CAS CSCD 北大核心 2009年第1期55-58,共4页 Guihaia
基金 科技部国际科技合作项目(2007DFA01240) 国家自然科学基金(30470030)~~
关键词 杜氏盐藻 核基质附着区 核基质结合区结合蛋白 真核表达载体 细胞定位 Dunaliella salina matrix attachment region MAR binding protein eukaryotic expression cellular localization
分类号 Q781 [生物学—分子生物学]
  • 相关文献

参考文献14

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二级参考文献15

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共引文献6

同被引文献15

  • 1王天云,侯卫红,袁保梅,柴玉荣,侯桂琴,王建民,薛乐勋.杜氏盐藻随机MAR文库的构建[J].实验生物学报,2005,38(1):23-28. 被引量：10
  • 2王亚峰,王天云,姬翔,刘红涛,王建民,薛乐勋.杜氏盐藻核基质附着区结合蛋白cDNA片段的克隆及序列分析[J].郑州大学学报（医学版）,2006,41(3):436-439. 被引量：3
  • 3关英芝,崔艳红,李晶,朱延明.MAR及其在转基因植物中的研究与应用[J].东北农业大学学报,2007,38(2):261-264. 被引量：2
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广西植物
2009年 第1期

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