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IGF-Ⅰ联合TGF-β1促成骨细胞的增殖和分化效应 被引量：3

Effects of IGF-Ⅰand TGF-β1 on proliferation and differentiation of human osteoblast-like cell
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摘要 目的探讨重组人胰岛素样生长因子-Ⅰ(rhIGF-Ⅰ)和重组人转化生长因子β1(rhTGF-β1)联合应用对成骨细胞增殖和分化的影响。方法①体外培养成骨细胞株MG-63,分组以不同浓度的IGF-Ⅰ(0.1、1.0、10.0和100.0ng/mL)或TGF-β1(0.01、0.1、1.0和10.0ng/mL)培养,MTT法检测细胞增殖,得到IGF-Ⅰ与TGF-β1单独作用的最适浓度。②选用最适浓度的IGF-Ⅰ,TGF-β1单独或联合培养,MTT法检测细胞增殖,ALP试剂盒检测细胞分化,分析两种细胞因子联合应用时,在成骨细胞增殖和分化过程中的作用。结果①IGF-Ⅰ与TGF-β1对成骨细胞MG-63均有一定的促增殖和促分化作用,促增殖作用以10ng/mL的IGF-Ⅰ在第3天,1ng/mL的TGF-β1在第5天最为显著。②在上述浓度联合刺激条件下,促增殖和促分化效果均优于单一的IGF-Ⅰ或TGF-β1单独应用组。结论IGF-Ⅰ与TGF-β1均有一定的促MG-63细胞增殖和分化效应,两者联合应用时具有良好的协同作用。 [ Objective] To investigate the effects of single and combined use of insulin-like growth factgor I （IGF- I ） and transforming growth factor β1 （TGF-β1） on the proliferation and differentiation of human osteoblast-like cell MG-63 cultured in vitro. [Methods] （1）MG-63 cells cultured in vitro were treated with different dosages of rhIGF- I （0.1, 1.0, 10.0 and 100.0 ng/mL） or rhTGF-β1 （0.01, 0.1, 1.0, 10.0 ng/mL）. Cell proliferation was measured by methylthiazol tetrazolium （MTF） colorimetrie method to choose an optimal dosage of rhlGF- I and rhTGF- β1. （2）MTT method and alkaline phosphatase （ALP） measurement kit were used to determine the proliferation at different time points （1, 3, 5 and 7 days） and ALP activity of MG-63 ceil after optimal dosage of rhIGF- I or/and rhTGF-β1 were added into the culture medium. [Results] （1）Compared with control group,both IGF- I and TGF-β1 significantly promoted the proliferation of MG-63 （P 〈0.05）. The cell number in osteoblasts exposed to IGF- I - treated cells was higher than that in TGF-β1-treated ceils （P 〈0.05）. （2）When using different dosages, 10 ng/mL IGF- I and lng/mL TGF-β1 showed the strongest effects of promoting proliferation （P 〈0.05）. （3）The combined group of IGF- I （10 ng/mL） and TGF-β1 （1 ng/mL） showed an even higher proliferative effect than using them respectively. （4）Compared with control group,significant changes of cellular ALP activities were found when using IGF- I or TGF-β1 while combining them showed stronger effects. [Conclusion] The combined use of IGF- I and TGF-β1 in a proper concentration signifieandy enhances the proliferation and differentiation of human osteoblasts cultured in vitro.
作者 黄生高 闫颖
机构地区 中南大学湘雅二医院口腔科
出处 《中国现代医学杂志》 CAS CSCD 北大核心 2009年第2期189-191,194,共4页 China Journal of Modern Medicine
关键词 成骨细胞 IGF—I TGF-Β1 细胞增殖 细胞分化 IGF- I TGF-β1 cell proliferation cell differentiation osteoblast
分类号 R336 [医药卫生—人体生理学]
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参考文献13

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共引文献17

同被引文献50

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引证文献3

二级引证文献16

中国现代医学杂志
2009年 第2期

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