摘要
目的探讨真皮模板单元－黏着斑微操控制对皮肤成纤维细胞生物学行为影响。方法采用微图案方法制作微凸阵列，运用分子自组装在微凸上自组装结构蛋白分子，在自制细胞培养仪模拟皮肤生物力学进行细胞牵拉2周，并行形态学观察和细胞免疫组织化学分析。结果细胞牵拉2周后，细胞形态多样、丰满，分泌增加，呈现顺应性。细胞免疫组织化学分析发现，整合素β1、α5表达较对照显著减少，张力蛋白tensin表达显著降低。结论通过黏着斑微操控，增生性瘢痕成纤维细胞在形态学及生物学行为上发生改变。
Objective To explore the effect of focal-adhesion micromanipulation on the biological behavior of fibroblast. Methods Micro-pot was made by microcontact printing. The molecules of constitutive protein was adhered on micro-pot by self-assemble of peptides. Skin fibroblasts were cultured on the membrane by self-made biomechanical cell culture for 2 weeks. Morphology observation and cell immunohistochemistry analysis was performed. Results After 2 weeks, the morphology of the fibroblasts was diverse and more compliant. Cell immunohistochemistry analysis found that the expression of integrinβ1, α5 and tensin was dramatically reduced. Conclusions The morphology and the biological behaviour of the fihroblasts in hypertrophic scar can be changed after micromanipulation of focal adhension.
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2009年第1期57-60,共4页
Chinese Journal of Plastic Surgery
基金
国家973项目（2005CB522603-3）
国家自然科学项目资助（30872685）
关键词
瘢痕
真皮模板单元
黏着斑
Cicatrix
Dermis template unit
Focal adhesion
