摘要
本研究以纯化的犬细小病毒(CPV)重组VP2蛋白为包被抗原,建立了检测CPV抗体的间接ELISA方法,其抗原包被浓度为0.158μg/mL;待检血清稀释倍数为1∶100,作用时间为60min;羊抗犬酶标抗体稀释倍数为1∶2000,作用时间为60min;底物作用时间为10min;判定标准为S/P≥0.282时为阳性,S/P≤0.226时为阴性。该方法与犬瘟热、犬传染性肝炎、犬副粘病毒病、犬波特氏杆菌病等4种犬常见传染病阳性血清无交叉反应,具有较好的特异性;批内、批间重复试验变异系数均小于15%,显示该方法具有良好的重复性;与商品化CPVELISA试剂盒进行比较,符合率为96.5%。本研究为现地免疫犬群抗体监测和进行CPV流行病学调查提供了一种简便的血清学诊断方法。
An indirect-ELISA was developed for detecting antibodies against canine parvovirus （CPV） using recombinant VP2 protein. The assay was highly specific and had no cross reaction with positive serum of canine parainfluza, canine bordetella bronchiseptica, canine distemper virus and canine hepatitis. The variation coefficients were less than 15 % in the intro-batch and in the inter-batch tests, indicating good interplate repeatability and intraplate reproducibility of the assay. There was 96.5 % agreement in comparison with the commercial kit. The indirect ELISA assay would provide a simple and rapid means for monitoring CPV infection or assessing vaccination in the field.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2009年第2期141-144,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
黑龙江省科技计划项目(PC07S010)
