摘要
目的探讨人与猪胆总管几何形态及顺应性的关系，为猪到人异种肝移植提供理论依据。方法取5例成年人尸体和50例3～12月龄湖北白猪的胆总管，在软组织生物力学实验机上测定胆总管的压力一直径关系，计算出顺应性。横断取材，冷冻切片，HE染色，用计算机图像分析系统测量其管径和管壁厚度。结果3～6月龄猪胆总管的管壁比成人的薄，其直径比成人的小（P〈0．01），7～10月龄猪胆总管的壁厚和直径与成人的相近（P〉0．05）。3～6月龄及11、12月龄猪胆总管的顺应性明显小于成人（P〈0．01），7～10月龄猪胆总管的顺应性与成人的相近（P〉0．05）。结论7～10月龄猪胆总管的几何形态和顺应性与成人的相近，从生物力学方面考虑，7～10月龄的猪肝作为人异种移植肝供源是可能的。
Objective To explore the relationship of the geometric morphology and compliance of the common bile duct （CBD） between human and pig and to provide theoretical basis for pig-to-human liver xenotransplantation. Methods The CBDs of 5 adult corpses and 50 Hubei white pigs aged between 3-12 months were obtained. The pressure-diameter relationship of the CBD was measured in the biomechanical testing system for soft tissues; the compliance was calculated then. The materials were extracted through transverse frozen sections, and then stained with HE. The diameter and wall thickness of CBD were measured by computer image analysis system. Results The diameter and the wall thickness of CBD of pigs aged between 3-6 months were significantly shorter or thinner respectively than that of adult human （ P 〈 0.01 ）, while the results of pigs aged between 7-10 months are similar to that of the adult human （ P 〉 0.05）. The compliance of the CBD of pigs aged between 3-6 months and 11- 12 months was significantly smaller than that of adult human （ P 〈 0.01 ）, while the results of pigs aged between 7-10 months were similar to that of the adult human （ P 〉 0.05）. Conclusion The geometric morphology and compliance of the CBD of pigs aged between 7-10 months are similar to that of the adult human, suggesting that it is possible for the liver of pigs aged between 7-10 months to be used as the donor for liver xenotransplantation according to biomechanics.
出处
《解剖学报》
CAS
CSCD
北大核心
2009年第1期164-167,共4页
Acta Anatomica Sinica
基金
湖北省教育厅科学技术研究项目(B20082410)
郧阳医学院创新团队项目(2008CXG02)资助
关键词
胆总管
几何形态
顺应性
生物力学
异种移植
人
猪
Common bile duct
Geometrical morphology
Compliance
Biomechanics
Xenotransplantation
Human
Pig
