摘要
目的研究淫羊藿黄酮类成分在Caco-2细胞模型中的吸收转运。方法采用超高压液相法测定药物浓度,计算表观渗透系数(Papp),研究淫羊藿黄酮5个主要成分淫羊藿苷、朝藿定A、朝藿定B、朝藿定C、宝藿苷Ⅰ在Caco-2细胞模型中的双向转运。结果淫羊藿苷、朝藿定A、朝藿定B、朝藿定C的吸收渗透系数(PAB)较小,分别为5.91×10-7、3.22×10-7、2.76×10-7、4.23×10-7cm/s,宝藿苷Ⅰ相对较大,为1.46×10-6cm/s;5个化合物的分泌渗透系数(PBA)都比其相应的吸收渗透系数要大,其中宝藿苷Ⅰ的分泌渗透系数是其吸收渗透系数的9.8倍。结论淫羊藿黄酮类成分的肠道吸收较差,可能存在肠道转运蛋白的外排机制,其中三糖苷(朝藿定A、朝藿定B、朝藿定C)的吸收要小于二糖苷(淫羊藿苷),二糖苷的吸收要小于单糖苷(宝藿苷Ⅰ)。
Objective To study the absorption and transportation of flavonoids in Herb Epimedii by using Caco-2 monolayer model. Methods Caco-2 cell monolayer model was used to study the bi-direction transport of icariin, epimedin A, epimedin B, epimedin C, and baohuoside I. The concentration of the five flavonoids in cell culture medium was measured by UPLC and the apparent permeability coefficients （Papp） were calculated. Results The absorptive permeability coefficients （PAB） of icariin, epimedin A, epimedinB, epimedinC, andbaohuoside I were 5.91×10^-7, 3.22×10^-7, 2.76×10^-7, 4.23×10^-7, and 1.46 × 10^-6 cm/s, respectively. Except baohuoside I , the other four flavonodes had lower permeabilities, and the secretive＇ permeabilities （PBA） of all the flavonoids were larger than their absorptive permeabilities. Among them, the PBA of baohuoside I was 9.8 times as much as the PAB. Conclusion The results suggest that the intestinal absorption of the five flavonoids is lower, which might have efflux mechanism by transporters, and the absorption of monloglycoside （e. g. baohuoside I ） is better than that of diglycoside （e. g. icariin） and triglycoside （e. g. epimedin A, epimedin B, and epimedin C）.
出处
《中草药》
CAS
CSCD
北大核心
2009年第2期220-224,共5页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(30572372)
