摘要
目的建立兔肾动静脉全阻断模型,研究肾缺血再灌注损伤的兔肾皮质内抗坏血酸浓度的实时变化规律。方法试验用白兔16只,其中实验组8只,对照组8只。实验组:麻醉后,手术分离出左肾及动静脉,将微透析探针沿肾长轴插入肾皮质内,平衡60min后接入实时电化学分析系统。正常血流灌注状态下测量肾内抗坏血酸基础值20min后将肾动静脉全部阻断,60min后开放动静脉,观测60min,实时监测整个过程肾透析液中抗坏血酸浓度的变化。同法处理对照组,对照组仅游离肾动、静脉而没有全阻断血管。结果实验组正常状态下透析液抗坏血酸浓度为(18.9±7.5)μmol/L,缺血期抗坏血酸平均浓度为(24.8±11.3)μmol/L,缺血期较自身正常灌注期平均升高31.2%[(24.8-18.9)/18.9]。再灌注期肾皮质透析液抗坏血酸浓度平均为(23.3±8.9)μmol/L,再灌注期较自身正常灌注期平均升高23.3%[(23.3-18.9)/18.9]。实验组内缺血期肾皮质抗坏血酸浓度较自身正常灌注期差异有显著性(P<0.05),与再灌注期差异无显著性(P>0.05)。结论抗坏血酸是反映肾缺血再灌注损伤的一个敏感指标,应用微透析-电化学法实时测定抗坏血酸水平可以实时反映肾皮质缺血状态,为研究肾缺血再灌注损伤建立良好的技术方法。
Objective To establish a model of renal artery and vein blockage and detect the real time change of ascorbic acid in the renal cortex in vivo. Methods Sixteen rabbits were randomly divided into experiment and control groups （8 in each）. Under anesthesia, the left kidney and artery-vein were dissociated and a microdialysis probe was inserted into the renal cortex. After being balanced for 60 minutes, the change of ascorbic acid in the renal cortex was detected. Ischemia was achieved by clamping the renal pedicle for 60 minutes followed by reperfusion for another 60 minutes in the experiment group. While in the control, the same procedure was performed during a same time interval without clamping the renal pedicle. Microdialysis samples were collected before, during, and after the ischemia in the rabbits, and then ascorbic acid in the microdialysates were real-time measured by an electrochemical method. Results The concentrations of ascorbic acid in the microdialysates were （ 18.9 ± 7.5 ） μmol/L, （24.8 ± 11.3） μmol/L, and （23.3± 8.9） μmol/L respectively before, during, and after the renal warm ischemia. In the experiment group, the concentration of ascorbic acid was increased rapidly by 31. 2% [ （24. 8 - 18.9 ）/18.9 ] following ischemia （ P 〈 0.05 ） ; no significant difference was found in the concentration of ascorbic acid between the base level and the value detected during reperfusion （P 〉 0.05 ）. Conclusions Ascorbic acid is a sensitive indicator of renal ischemia/reperfusion. The level of ischemia in renal context can be monitored in real time by detecting the concentration of ascorbic acid in vivo using microdialysis-electrochemical method. The method can be used for the further studies on ischemia/reperfusion.
出处
《中国微创外科杂志》
CSCD
2009年第1期69-72,共4页
Chinese Journal of Minimally Invasive Surgery
关键词
抗坏血酸
微透析
肾缺血再灌注损伤
Ascorbie acid
Microdialysis
Ischemia/Reperfusion injury
