摘要
目的评价染色体核型分析联合骨髓活检在诊断无明显病态造血的早期骨髓增生异常综合征(MDS)中的价值。方法回顾性分析9例有克隆性染色体异常的无病态造血改变的早期MDS的染色体核型和骨髓活检组织切片特征。结果9例MDS患者中,8例为轻度的异常核型,1例为复杂核型。4例有+8异常,2例有13、20号染色体异常,9、10、11、12、14号染色体受累各1例。骨髓切片CD61免疫组化示2例有微小巨核阳性,检出1例典型髓系幼稚前体细胞异常定位(ALIP),3例患者骨髓切片CD34+细胞占骨髓有核细胞数的比例较缺铁性贫血骨髓切片(8例对照)的CD34+细胞有增高趋势,但该3例患者骨髓涂片CD34+细胞并未显示增多。结论联合应用染色体核型分析和骨髓活检有助于对没有明显病态造血改变的早期MDS进行诊断。
Objective Objective To investigate the value of the combined tests with clonal chromosomal abnormalities and marrow biopsy for diagnosing the early myelodysplastie syndromes without evidence of dysplastie hematopoiesis.Methods Clonal chromosomal karyotype and bone marrow biopsy analysis were performed retrospectively in 9 early MDS cases with the absence of dysplastic features.Results 8 of the 9 MDS patients had simple clonal cytogenetic abnormalities,only one case had a complex karyotype.Four patients in this series had trisomy 8, abnormalities of chromosomes 13,20 were found respectively in two cases, chromosomes 9, 10, 11,12 and 14 was involved one time respectively. The results of marrow biopsy in 9 cases showed that 2 cases had CD61-positive small -micro megakaryocytes,1 case had an atypical abnormal localization of immature precursors（ALIP）.3 BM biopsies demonstrated a slight increase tendency in CD34-positive cells as compared to what is observed in IDA BMs, but no increased blast population was seen in the BM aspirates of theses cases. Conclusion combined tests with chromosomal karyotype and marrow biopsy may be useful for diagnosing the early MDS without evidence of dysplastic hematopoiesis.
出处
《中国实验诊断学》
北大核心
2009年第2期241-244,共4页
Chinese Journal of Laboratory Diagnosis
