摘要
目的:构建湖北恩施产厚朴的ISSR指纹图谱。方法:采用ISSR标记技术对厚朴基因组DNA进行PCR扩增。结果:2个ISSR引物即可构建恩施产厚朴的ISSR指纹图谱。引物ISSR826的扩增产物中可见构建恩施产的厚朴特征带,分子量为950 bp及550 bp。结论:ISSR标记技术可用于区分厚朴药材产地和品种。
Objective ： To establish the ISSR fingerprint of the cortex of Magnolia officinalis produced from Enshi. Methods ： Cuhivar and habit identification was based on Inter-simple sequence repeats（ISSR）. Results： Two ISSR primes could be used to construct the ISSR fingerprint of M. officinalis from Enshi. Conclusion：The ISSR marker is an effective method for the cultivar and habit identification of the cortex of M. officinalis.
出处
《中药材》
CAS
CSCD
北大核心
2009年第1期19-22,共4页
Journal of Chinese Medicinal Materials
关键词
厚朴
ISSR
指纹图谱
Magnolia officinalis Rehd. et Wils.
ISSR
Fingerprint
