摘要
目的研究T淋巴瘤侵袭转移诱导因子1(Tiam1)和血管内皮生长因子(VEGF)在非小细胞肺癌(NSCLC)中的表达及其与癌组织中微血管密度(MVD)的关系,并探讨它们与NSCLC生物学行为的关系。方法选用手术切除并经病理学确诊的71例NSCLC癌组织和16例肺良性病变组织,应用免疫组织化学S-P法检测组织中的Tiam1和VEGF的表达,并对CD34表达阳性血管进行微血管密度计数(MVO)。结果Tiam1和VEGF在71例NSCLC中阳性表达率分别为60.6%、67.6%,在肺良性病变阳性表达率分别为6.3%、18.8%,两者在NSCLC中的阳性表达率高于肺良性病变组织(P<0.05);Tiam1、VEGF在NSCLC组织中的表达与临床分期和脑转移有良好的相关性(P<0.05),与年龄、性别、病理类型和分化程度无关(P>0.05);NSCLC和肺良性病变的MVD值分别为47±10和13±5,差异具有统计学意义(P<0.05)。结论Tiam1、VEGF参与了NSCLC的发生发展过程,检测Tiam1和VEGF的表达可以作为判断NSCLC的血管生成、侵袭、脑转移等生物学行为的指标,两者有望成为非小细胞肺癌新的治疗途径。
Objective To investigate the expression of Tiam1 （T-lymphoma invasion and metastasis inducing factor 1） and VEGF （vascular endothelial growth factor） in human non-small cell lung cancer（NSCLC） tissues and their relationship with microvascular density（MVD） in cancer tissues and with the biological behaviour of NSCLC. Methods The specimens were collected from 71 patients with NSCLC and 16 cases of benign lesions of lung who were diagnosed pathologically and operated at the department of cardio-chest surgery, lmmunohistochemical S-P technique was used to detect the expression of protein of Tiam1 and VEGF. The microvessel density in tumor was counted by immunostaining with CD34. Results The positive rate of Tiam1 and VEGF expression in non-small cell lung cancer was 60.6% and 67.6% respectively, and which was 6.3% and 18.8% in lung benign lesions respectively, and the positive rates of expression of both factors in non-small cell lang cancer were significantly higher than those in lung benign lesions（ P 〈 0.05）. The expression of Tiam1 and VEGF was closely related to clinical stage and brain metastasis of non-small cell lung cancer （ P 〈 0.05）. But the expression of Tiam1 and VEGF was not correlated with age, sex, histology and differentiation of non-small cell lung cancer （ P 〉 0.05）. The value of MVD of non-small cell lung cancer and lung benign lesion was （47 ± 10） and （13 ± 5） respectively, and there was a significant difference between the two groups （P 〈 0.05） .Conclusion Tiam1 and VEGF are more highly expressed in non-small cell lung cancer than those in lung benign lesion tissues, and they were positively correlated with brain metastasis and clinical pathological stage. The expression of Tiam1 and VEGF is positively correlated （ r = 0.550, P 〈 0.05）. Thus the high expression of Tiam1 and VEGF is involved in non-small cell lung cancer development. Tiam1 and VEGF can be used as a major marker for detecting biological behavior and prognosis of non-small cell lung cancer to judge the angiogenesis, invasion and brain metastasis prognosis of non-small cell lung cancer, and they can be used as a new approach to treat non-small cell lung cancer.
出处
《河北医药》
CAS
2009年第2期134-136,共3页
Hebei Medical Journal
关键词
T淋巴瘤侵袭转移诱导因子1
血管内皮生长因子
CD34
非小细胞肺癌
T-lymphoma invasion and metastasis inducing faetorl
vascular endothelial growth factor
CD34
non-small cell lung cancer
