摘要
目的研究PI3K／PTEN／AKT／mTOR信号通道在胃癌组织中的表达及临床意义。方法采用免疫组化sP法分别检测33例胃癌及30例正常胃组织中mTOR、FFEN的表达情况，并分析二者与性别、年龄、淋巴结转移、浸润深度和分化程度的关系。结果mTOR蛋白阳性反应产物主要分布于胞质，PTEN的蛋白阳性反应产物主要定位于胞核。将mTOR表达阴性，PTEN表达阳性为一组，与mTOR表达阳性而PTEN表达阴性为一组进行比较，发现对于不同的大体类型，组织学分型，二组间的差异有统计学意义（P〈0．05），对于不同的淋巴结转移，临床病理分期，二组问的差异有统计学意义（P〈0．01）。结论在PI3K／PTEN／AKT／mTOR信号通道中，mTOR与PTEN的联合表达可作为判断胃癌分期、判断预后的指标和化学治疗的靶点。
Objective To study the expression and clinical significance of the signal pathway PI3K/PTEN/AKT/mTOR in gastric carcinoma tissues. Methods The expression of mTOR and PTEN in 33 cases of gastric carcinoma and 30 cases of normal gastric tissue was detected by immunohistochemistry SP method respectively, and its relation with gender, age, tumor metastasis, the depth of invasion and differentiation analyzed. Results The positive product of the mTOR protein was mainly distributed in the endochylema, while the positive product of the PTEN protein mainly located in the nucleus. We regarded the negative roTOR with positive PTEN as one group, and negative PTEN with positive mTOR as the other group. It was found there was significant difference in different categories and histological types, different metastases, and clinical pathology stage between the two groups （ P 〈 0.01 or 0.05 ）. Conclusion The expression of roTOR and PTEN is closely related in the signal pathway PI3K/PTEN/ AKT/mTOR. The combined expression of mTOR and PTEN can be used to justify staging of gastric carcinoma and the prognosis, and as a target of the chemotherapy.
出处
《临床外科杂志》
2009年第2期106-108,共3页
Journal of Clinical Surgery
基金
湖北省科技攻关项目（2005AA304804）
