摘要
目的:探讨理气开郁法四个小复方对慢性应激大鼠胃和下丘脑P物质的影响。方法:采用多因素随机性反复刺激引起慢性应激大鼠模型,再分别使用四个理气开郁小复方及阳性对照药进行治疗。应用放射免疫法测定组织匀浆中的P物质。结果:慢性应激大鼠出现类似肝郁脾虚的表现。与正常组大鼠比较,模型组大鼠胃和下丘脑SP含量显著降低(P<0.05);13.5g/kg痛泻要方可提高胃组织SP,且作用强于4.5g/kg痛泻要方和氯米帕明(P<0.05);13.5g/kg会铃子散的作用也强于氯米帕明(P<0.05)。13.5g/kg柴郁汤能提高下丘脑SP,且作用强于1.5g/kg柴郁汤(P<0.05)。结论:采用多因素随机性反复刺激引起慢性应激大鼠胃和下丘脑SP水平降低,痛泻要方提高胃组织SP的作用较强,柴郁汤提高下丘脑SP的作用较强。
Objective To investigate the effects of Chaiyu Decoction, ShaoganDecoction, Jinlingzi powder and Tongxie prescription on the level ofSubstance P in the stomach and hypothalamus of chronic psychological stress rats. Methods A rat model of chronic stress was made by multi - random factors tostimulaterepeatedly. Substance P in the tissusbemogenate was determined using radioimmunoassay. Results Chronic psychological stress rats had an expression of liver depression and deficiency. The level of Substance P in the stomach and hypothalamus of the model group rats were significantly lower than those in normal group. （ P 〈 0.05）. 13.5g/kg Tongxie prescription could increase Substance P in the stomach and hypothalamus, and had a better effect than 4.5g/kg Tongxie prescription, and Clomipramine. （ P 〈 0. 05）. 13.5g/kg Jinlingzi powder had a better effect than C lomipramine. （ P 〈 0.05）. 13.5g/kg Chaiyu Decoction can increase the level of substance P in the hypothalamus, and had a better effect than 1.5 g/kg ChaiyuDecoction, （ P 〈 0.05） Conclusion The levels of substance P in the stomach and hypothalamus of the chronic psychological stress rats were decreased . Tongxie prescription can significantly increase the level of Substance P in the stomach, and Chaiyu Decoction can make a significant effect on Substance P in the hypothalamus.
出处
《中国中西医结合外科杂志》
CAS
2009年第1期77-80,共4页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
基金
天津市科技攻关计划重大科技项目(05YFGDSF02600)分项目(按治则归类进行新药开发与作用机理研究)
关键词
多因素随机性慢性应激
P物质
痛泻要方
柴郁汤
余铃子散
芍药甘草汤
下丘脑
胃
multi-random factors of chronicSubstance P, ChaiyuDecoction, Shaogan Decoction,powder, Tongxieprescription, stomach , hypothalamus stress,Jinlingzi
