摘要
作为一种新型的细胞分子遗传学技术,荧光原位杂交(FISH)是利用荧光标记DNA探针来检测细胞内染色体异常的非放射性原位杂交技术,可检测基因缺失、扩增、异位等异常,并结合组织形态学,提高病理诊断的准确性。文中主要综述其在实体瘤诊断方面的一些应用。
Fluorescence in situ hybridization （FISH） is a non-radioactive technique by use of fluorescently labeled DNA probes in detecting chromosomal alterations in cells. As a new molecular cytological method, it can detect various types of cytogenetic alterations, including deletion, amplification and trans- location, as well as improve the accuracy of pathological diagnosis if combined with histomorphology. This review focuses on the application of FISH to the detection of some solid tumors.
出处
《医学研究生学报》
CAS
2009年第2期213-216,共4页
Journal of Medical Postgraduates
基金
南京军区医学科学技术研究"十一五"计划课题基金资助(批准号:06Z37)
关键词
荧光原位杂交
FISH
肿瘤
诊断
Fluorescence in situ hybridization
Tumor
Diagnosis
