摘要
目的探讨p27蛋白在Ⅱ期结肠癌组织中的表达意义。方法应用免疫组织化学方法结合组织芯片技术检测北京肿瘤医院57例Ⅱ期结肠癌组织中p27蛋白的表达情况，采用Kaplan-Meier法进行生存分析，Pearson X^2检验其相关性，Cox比例风险回归模型进行多因素分析。结果在Ⅱ期结肠癌中p27蛋白的高表达率为60％（34／57），与结肠癌的分化程度、肿瘤部位、远处转移有关（X^2=5．97，5．93，5．05，P〈0．05）；与患者年龄、肿瘤大小、生长方式等无关（X^2=0．64，0．49，0．33，P〉0．05）；p27蛋白高表达患者5年生存率为94％（32／34），明显高于低表达患者的78％（18／23）（X^2=3．86，P〈0．05）。多因素分析显示：p27蛋白低表达和肿瘤穿透浆膜累及癌周是Ⅱ期结肠癌的独立不良预后因素。结论p27蛋白低表达是Ⅱ期结肠癌的独立不良预后因素之一，可以为临床筛选高危Ⅱ期结肠癌提供重要参考依据。
Objective To investigate the expression and clinical significance of p27 protein in stage Ⅱ colon cancer. Methods The expression of p27 protein in 57 samples of stage Ⅱ colon cancer tissue from Beijing Cancer Hospital was detected by immunohistochemistry and tissue microarray technique. Survival was analyzed by the Kaplan-Mcier method, and the correlation between the expression of p27 protein and the prognosis of patients was analyzed by Pearson chi-square test. Multivariate analysis was done by using Cox proportional hazard regression model. Results The rate of high expression of p27 protein in stage Ⅱ colon cancer was 60% （34/57）. The differentiation, location and distal metastasis of the tumor were correlated with the expression of p27 protein （ X^2 = 5.97, 5.93, 5.05, P 〈 0.05 ） ; while age, tumor size, growth patterns of tumor were not correlated with the expression of p27 protein （ X^2 = 0. 64, 0.49, 0.33, P 〉 0.05 ）. The 5-year survival rate of the patients with high expression of p27 protein was 94% （32/34）, which was significantly higher than the 78% （ 18/23 ） of the patients with low expression of p27 protein （ X^2 = 3.86, P 〈 0.05 ）. Multivariate analysis showed that low expression of p27 protein and tumor penetrating serous membrane are the independent adverse prognostic factors for stage Ⅱ colon cancer. Conclusions Low expression of p27 protein is one of the independent adverse prognostic factors for stage Ⅱ colon cancer, and the expression of p27 protein should be consulted when screening out the patients who are in the high risk group of stage Ⅱ colon cancer.
出处
《中华消化外科杂志》
CAS
CSCD
2009年第1期57-59,共3页
Chinese Journal of Digestive Surgery
基金
基金项目：科技部“十一五”支撑计划（2008BA152B03）
关键词
结肠肿瘤
Ⅱ期
P27蛋白
预后
Colonic neoplasms
Stage Ⅱ
p27 protein
Prognosis
