摘要
目的:建立不同产地小花鬼针草药材的毛细管指纹图谱相似度的研究方法。方法:采用毛细管区带电泳法,以50 mmol·L-1硼砂水溶液-100 mmol·L-1硼酸水溶液(2∶1)为背景电解质(BGE)溶液,运行电压20 kV,紫外检测波长254 nm,流体力学进样10 s(压力,5×103Pa);记录小花鬼针草的毛细管电泳特征图谱;并以"中药色谱指纹图谱相似度评价系统(2004 A版)"相似度计算软件和"SPSS计算软件"进行评价。结果:将28批药材分为3类,其分类情况基本与产地相符合,在方法的精密度、重复性及稳定性试验中,各特征峰的相对迁移时间的RSD小于6%,相对峰面积的RSD小于10%。结论:2种评价方法的结果基本一致,说明本方法能够有效评价小花鬼针草药材的相似性,由分类结果可以看出,不同产地的药材存在差异性。
Objective： To develop a method to study the specific chromatogram similarity of Bidens parviflora Willd. from different area by HPCE. Method： The background electrolyte （BGE） was a 0. 05 mol·L^-1 sodium borate solution （add 50% 0. 10 mol·L^-1 borate solution）;The UV detection wavelength was 254 nm and a voltage of 20 kV was applied;And evaluated with the similarity evaluation system for chromatographic fingerprint of TCM （Version 2004 A） and the evaluation system of SPSS. Results： The 28 batches were divided into 3 types, which perfectly consistent with the distribution by areas;The CE specific chromatogram had acceptable precision and reproducibility with the RSD of the relative migration time less than 6% and the RSD of the relative peak area less than 10%. Conclusions ：The evaluations by two different methods were similar, and from which we can speculate that this method can effectively evaluated the similarity of Bidens parviflora Willd. from different areas.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第2期179-183,共5页
Chinese Journal of Pharmaceutical Analysis
基金
深圳市2005年自然科学基金资助项目
关键词
小花鬼针草
毛细管区带电泳
特征纹图谱
Bidens parviflora Willd.
capillary electrophoresis （CE）
specific chromatogram
