摘要
利用玉米浸泡水生产酵母,将培养基pH调至4.0后在未灭菌的情况下接入酵母培养,摇瓶培养酵母菌,湿菌体量可达约10g/100mL,发酵罐培养可达20～30g/100mL。将培养好的酵母添加到已调至pH4.0的赖氨酸发酵液中,非无菌条件下再发酵约6h,降低了赖氨酸发酵液残糖,降低了发酵液黏度。该工艺不仅解决了玉米浸泡水的污染问题,同时也极大地改善了赖氨酸下游工艺,取得了良好的经济效益和社会效益。
In order to use corn steep liquor to produce yeast, we cultured the yeast using desterilized culture media after adjusting pH of culture media to 4.0, approximately 10g/100mL wet biomass of yeast were achieved when cultured by flask shaking, while 20-30 g/100mL wet biomass of yeast were gained by culture in fermentor. Adding the yeast just cultured into the lysine broth which had just been adjust to pH4.0, then continue fermentation about 6h under sterilized conditions, after that, the residue glucose of the lysine broth were decreased and the viscosity of the lysine broth were reduced. This finding not only provides a solution to the pollution problem of corn steep liquor but also improves lysine production progress drastically, and likely induces economic benefit and social benefit.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2009年第1期103-105,共3页
Food and Fermentation Industries
