摘要
基因进化的研究和重构通常是在序列水平上进行的,包括比对它们的基因序列或蛋白序列.而对基因外显子/内含子结构的分析能够提供更多有价值的信息,比如绘制更为可靠的系统发生图谱,或更精确地阐明内含子的进化.为此,本文设计了相应的Perl脚本程序来提取、比较和搜索基因说明文档中CDS..join特征域的Exon/Intron结构.通过该方法,可构建相关物种的Exon/Intron数据库(EID),其主要内容包括内含子的相位,Exon或Intron的数量和大小,剪接位点的模式以及选择性剪接(Alternative splicing,AS)的相关信息.
The study and reconstruction of gene evolution are usually conducted at the sequence level, including comparing and aligning their genomic, transcript or protein sequences. However, involvement of analysis of the exon - intron structure of genes may provide further and useful information, to draw reliable phylogenetic relationships unsolved by the traditional sequence -based evolutionary studies, or to shed more light on the patterns of the gain and loss of introns. In this work, a Perl program, designed to retrieve, compare and search for the exon - intron structure in CDS.. join features in the existing gene annotations, is presented. By this way, an Exon -Intron database （EID）, including the phase of intron, the number/size of exon and intron, the patterns of splicing site and the information of alternative splicing （AS） , can be constructed.
出处
《华南师范大学学报（自然科学版）》
CAS
北大核心
2009年第1期91-94,共4页
Journal of South China Normal University(Natural Science Edition)
基金
国家自然科学基金资助项目(30470495)
