摘要
目的:探讨血必净对大鼠外伤性脑损伤的神经保护机制。方法:将40只健康成年雄性SD大鼠随机分成4组:正常对照组、血必净治疗组、胞二磷胆碱治疗组和生理盐水组。采用改进的Feeney氏法制作大鼠自由落体脑创伤模型。伤后第6小时、第24小时、第3天、第5天、第7天断尾采血,运用双抗体夹心ELISA方法检测各组治疗后血清中S100B蛋白和白细胞介素-6(interleuk in-6,IL-6)的含量。结果:与正常对照组相比,血必净治疗组、胞二磷胆碱治疗组和生理盐水组血清中S100B蛋白和IL-6的含量均显著升高(P<0.001);与生理盐水组相比,血必净治疗组和胞二磷胆碱治疗组血清中S100B蛋白和IL-6的含量显著降低(P<0.001);与胞二磷胆碱治疗组相比,血必净治疗组血清中S100B蛋白和IL-6的含量显著降低(P<0.05)。结论:血必净可能通过抑制脑外伤后S100B和IL-6的合成与分泌,降低全身和局部的炎症反应程度,从而发挥神经保护作用。
Objective： To explore the neuroprotective mechanisms of Xuebijing on rats after traumatic brain injury.Methods： Forty healthy adult male Sprague-Dawley rats were randomly devided into 4 groups： normal control,Xuebijing,nicholin and saline groups.The model was made by improved Feeney′s traumatic brain injury model.Serum samples were collected at 6h,24h,3d,5d,7d after trauma.The serum S100B protein and IL-6 content of each group after treatment was detected with double antibody Sandwich ELISA. Results： Compared with the normal control group,the serum S100B protein and IL-6 content significant difference in Xuebijing,nicholin and saline groups（P〈0.001）;compared with the saline group,the serum S100B protein and IL-6 content significantly lower in Xuebijing and nicholin groups（P〈0.001）;compared with the nicholin group,the serum S100B protein and IL-6 content significantly lower in Xuebijing group（P〈0.05）. Conclusion： Inhibition of the composition and secretion of S100B protein and IL-6 by Xuebijing might be one of the mechanisms in decreasing inflammatory reaction systemic and local,and this may be how Xuebijing exerts its neuroprotective effect on traumatic brain injury.
出处
《江苏大学学报（医学版）》
CAS
2009年第1期35-38,共4页
Journal of Jiangsu University：Medicine Edition
