摘要
目的建立纳米粒沉淀法制备阳离子PLA-PEG纳米粒的方法。方法采用单因素设计考察不同影响因素对纳米粒粒径大小的影响,在单因素考察的基础上采用正交设计优化处方,制备了粒径较小,正电荷适中的阳离子PLA-PEG纳米粒。并对纳米粒的物理性质如物理形态,平均粒径,粒度分布,Zeta电位,DNA结合率,体外细胞转染能力等进行了考察。结果采用纳米粒沉淀法,通过优化处方和工艺制得的纳米粒外观圆整,呈类球形,大小均匀,平均粒径为89.7 nm,粒径分布指数为0.185,表面荷较高的正电荷,Zeta电位为+28.9 mV,能够高效的结合DNA且能够成功的转染Hela细胞。结论优化确定了纳米粒沉淀法制备阳离子PLA-PEG纳米粒的处方和工艺,可以制备满足细胞转染要求的阳离子载基因纳米粒。
Purpose To develop a nanoprecipitation method for preparation of cationic PLA-PEG nanoparticles（PLA-PEG-NPs）. Methods The effects of different formulations and technical factors on the particle size of nanoparticles were investigated by single factor investigation. Based on single factor investigation, the formulation was optimized by orthogonal design. The optimal nanoparticles were characterized in terms of morphology, average particle size, polydispersion index （PDI）, Zeta potential, DNA binding efficiency and in vitro cell transfection capability. Results The nanoparticles prepared by nanoprecipitation method were spherical in shape with average particle size of 89.7 nm, uniform in size with PDI of 0. 185, and positive in surface with Zeta potentials of ＋ 28.9 mV. The obtained PLA-PEG-NPs could bind efficiently with DNA onto its sur-face and successfully transfer Hela cells. Conclusion A nanoprecipitation method was optimized to manufacture gene loaded cationic PLA-PEG nanopartieles with excellent characteristics meeting cell transfection requirements.
出处
《中国生化药物杂志》
CAS
CSCD
北大核心
2009年第1期1-5,共5页
Chinese Journal of Biochemical Pharmaceutics
基金
国家自然科学基金资助项目(30572267)
