摘要
用3个能量(3.0、5.0和7.0MeV)和4个注量(1×1081、×109、1×1010和1×1011H+/cm2)的质子束处理大豆品种绥农14的风干种子,以期确定质子束诱变大豆的效果和辐照参数。在本试验条件下,经质子束辐照的大豆种子发芽率未受影响,但M1代株高显著增加;M2代出现早熟、黄化和不育等变异类型,总变异频率为6.81%,总变异频率且有随注量增加而增加的趋势,但随能量变化的规律不明显。在M2代,以3.0MeV1、×1011H+/cm2处理的变异频率最高,为8.26%;在不同能量和注量水平下得到的突变类型不同,其中质子注量为1×1010和1×1011H+/cm2的处理产生了较多早熟突变,注量为1×109和1×1010H+/cm2的处理产生了较多黄化和不育的突变;质子能量为3.0MeV时产生了较多的不育突变,5.0MeV时产生了较多的早熟突变,7.0MeV时产生了较多的黄化突变。结果表明质子束辐照能导致大豆产生丰富的变异,可以作为一种新的育种手段在大豆上应用。
To identify mutation inducing effects and parameters for irradiation treatment, three energy （3.0, 5.0 and 7.0MeV） and four integral fluxes levels （ 1×10^8、1×10^9、1×10^10 and 1×10^11H^＋/cm^2 ） of proton beam were applied to irradiate the dry seeds of soybean [ Glycine max （L.） Merr. ]cv. Suinong 14. The results showed that various mutations could be induced. Seed germination was not affected in the current experiment, whereas some other traits of the progenies were influenced, i.e., the height of MI plants was significantly increased, and mutants including early-maturing, etiolated, sterile types were found in M2 plants. Total mutant frequency was 6.81% and it increased with the increment of the fluence level but not the energy level. Mutant percentage of the M2 plants derived from the treatment of 3.0MeV, 1×10^11H^＋/cm^2 was 8.26 %, the highest among the 13 treatments. Mutation types varied according to the energy and fluency levels. Early-maturing mutants were preferentially achieved when irradiated with 1×10^10 or 1×10^11H^＋/cm^2 integral flux or with 5.0MeV proton beam, etiolated and sterile mutants were frequently obtained when treated with 1×10^9 or 1×10^10H^＋/cm^2 irradiation. When the seeds were irradiated with the energy of 3.0MeV or 7.0MeV, more sterile mutants and etiolated mutants were obtained than in other treatments. It was drawn that proton beam as a new mutagen can be used in soybean breeding.
出处
《核农学报》
CAS
CSCD
北大核心
2009年第1期49-53,共5页
Journal of Nuclear Agricultural Sciences
基金
“973”(2002CB111300)
“863”(2006AA100104-9)
国家科技支撑课题(2006BAD01A04)
关键词
大豆
质子束
能量
注量
诱变
soybean
proton beam
energy
integral fluxes
mutagenic effect
