摘要
在研究大豆遗传多样性的过程中，为了获得清晰、重复性好的SSR扩增结果，DNA的提取起着决定性的作用。以大豆干种子为实验材料，采用改良的SDS法对大豆DNA进行提取。试验结果表明，该改良方法所提DNA凝胶条带清晰，无拖尾现象，OD260／OD280检测值在1．7～1．9之间，用于PCR结果可靠、理想。
In the study of the soybean genetic diversity, in order to obtain a clear, reproducible results of the SSR amplification, DNA extraction played a decisive role. Soybean seed to dry for the experimental materials, use of improved soybean SDS method for DNA extraction. The results showed that the modified DNA gel method mentioned in the article with clear, non-tailing phenomenon, OD260/OD280 detected in the value between 1.7 to 1.9 for reliable PCR results of the ideal.
出处
《辽宁农业科学》
2009年第1期49-50,共2页
Liaoning Agricultural Sciences
基金
辽宁省自然科学基金项目
