摘要
目的:探讨半夏醇提物对人肝癌细胞(HepG2)的抑制作用及其机制。方法:用半夏醇提物(PTE)与体外培养的人肝癌HepG2细胞共培养,采用MTT比色法测定不同剂量组PTE对HepG2细胞生长的影响;采用流式细胞术分析PTE对其细胞周期分布的影响;免疫细胞化学法观察细胞周期调控相关蛋白cyclin D1、c-myc和β-catenin的表达。结果:PTE作用24 h、48 h和72 h对HepG2细胞的增殖均有显著抑制作用(P<0.05),随剂量增加抑制作用增强,其中400μg/mL PTE作用72 h,其抑制率达54.7%;PTE使HepG2细胞阻滞于G0/G1期,400μg/mL剂量组的比例达到(66.5±2.1)%,与对照组(58.6±1.3)%相比有显著性差异(P<0.01);PTE还使cyclin D1、c-myc和β-catenin蛋白表达显著下降,与对照组相比其阳性面积百分比和光密度值均有显著性下降(P<0.05)。结论:PTE可抑制HepG2细胞的增殖及周期调控相关蛋白cyc-lin D1、c-myc和β-catenin的表达,并使HepG2细胞周期阻滞于G0/G1期。
Aim： To investigate the effects of the ethanolic extract from PineUia ternate （PrE） on HepG2 cells in vitro. Methods： The human hepatoma HepG2 cells were treated with different concentrations of PTE. The effect of different concentrations of PTE on cell growth of HepG2 was measured by MTT. Its effect on cell cycle distribution of HepG2 was detected by flow cytometry. And the effects on the expression of cyclin D1, c-myc and β-catenin were assayed by immunocytochemistry. Results： PTE significantly inhibited the growth of HepG2 cells after 24 h, 48 h and 72 h treatment in a dose-dependent manner （P 〈 0. 05）. The inhibition rate reached 54. 7% after being treated with 400 μg/mL of PTE. The cell proportion of HepG2 at G0/G1 phase in 400 μg/mL PTE-treated group was significantly up-regulated （ 66. 5 ± 2. 1） % compared with that of the control （ 58.6 ± 1.3） % （ P 〈 0. 01）. Moreover, the expressions of cyclin D1, c-myc and β-catenin of PTE treated ceils were significantly lower than that of the control （P 〈0. 05）. Conclusion： PTE can induce the human hepatoma HepG2 cells arrested at G0/G1 phase, reduce the expression of cyclin D1, c-myc and β-catenin and inhibit cell proliferation.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2009年第1期89-93,共5页
Journal of China Pharmaceutical University
关键词
半夏醇提物
HEPG2
增殖
流式细胞术
免疫细胞化学
ethanolic extract from Pinella ternate
HepG2
proliferation
flow cytometry
immunocytochemistry
