摘要
目的研究亚慢性染铝对断乳大鼠神经行为学和神经细胞形态学的影响,以明确铝对发育中的中枢神经系统的毒性作用,为机制研究提供理论依据和实验资料。方法对刚断乳Wistar大鼠通过饮水亚慢性连续染毒12周,采用原子吸收石墨炉法进行血铝和脑铝含量的测定;跳台试验法观察大鼠学习、记忆行为学的改变;HE染色和尼氏染色后,光镜观察海马形态学改变。结果高剂量染铝组脑组织系数显著低于对照组;随着染铝剂量的增加,染铝组血铝和脑铝浓度显著升高(P<0.05,P<0.01),低、高剂量组之间脑铝差异有统计学意义(P<0.05);大鼠跳台试验的潜伏期随铝暴露剂量增加逐渐缩短,而错误次数逐渐增加,有明显的剂量-效应关系(P<0.01)。海马HE和尼氏染色后光镜观察表明,各染铝组与对照组相比均未发现明显差异。结论亚慢性铝暴露可以引起发育中大鼠学习记忆能力下降,但其形态学观察未见器质性病变。
Objective To explore the effects of subchronic exposure to aluminium on neural behavior and hippocampal morphology in weaning rats. Methods Weaning Wistar rats were randomly divided into three groups according to the body weight. Aluminium chloride was administrated through drinking water with the concentrations of 0. 2% and 0. 4% （m/V） respectively for 12 weeks. The blood levels of aluminium were determined by atomic absorption spectrophotometry, the neural behavior was examined by step down test, and the hippocampal morphological changes were observed under light microscope with HE and Nissl＇s stainings. Results The brain coefficients of exposed rats was remarkably lower than that of the controls and dose-dependent. The levels of aluminium in both blood and brain was increased significantly along with the administered dosage （P 〈 0. 05, P 〈0.01 ）. On the other hand, the latent period of step down test in exposed rats was shortened, while the mistake frequency was increased significantly along with the increase of aluminium dosage （P 〈 0. 01 ）. But no obvious morphological chan- ges in the hippocampi of exposed rats was found. Conclusions There were some adverse effects on learning and memory abilities by subchronic exposure to aluminium in the weaning rats, but no morphological change could be found in the study.
出处
《中国工业医学杂志》
CAS
北大核心
2009年第1期10-12,共3页
Chinese Journal of Industrial Medicine
基金
国家自然科学基金项目(No.30700675)
关键词
铝
亚慢性
学习记忆
形态学
Aluminium
Subchronic exposure
Ability in learning and memory
Morphology
