摘要
目的观察艾灸对溃疡性结肠炎肠纤维化大鼠结肠成纤维细胞(conlonic fibroblast,CFB)细胞内Ⅰ、Ⅲ、Ⅳ型胶原合成的影响,探讨艾灸防治溃疡性结肠炎肠纤维化的作用机制。方法采用免疫法加局部刺激制备溃疡性结肠炎肠纤维化大鼠模型,随机将大鼠分为正常组、模型组、隔药灸组、温和灸组和柳氮磺胺吡啶(SASP)灌胃组。隔药灸组、温和灸组选取天枢和气海穴,分别进行隔药灸、温和灸治疗;SASP灌胃组给予柳氮磺胺吡啶灌胃。分离并培养各组大鼠结肠成纤维细胞,Western印迹法观察艾灸对溃疡性结肠炎肠纤维化大鼠结肠成纤维细胞细胞内Ⅰ、Ⅲ、Ⅳ型胶原合成的影响。结果溃疡性结肠炎纤维化大鼠结肠成纤维细胞细胞内Ⅰ、Ⅲ、Ⅳ型胶原合成显著增强;隔药灸组、温和灸组大鼠结肠成纤维细胞细胞内Ⅰ、Ⅳ型胶原的合成均被显著抑制;SASP灌胃组大鼠结肠成纤维细胞细胞内Ⅰ型胶原的合成被显著抑制。结论艾灸能够调节溃疡性结肠炎肠纤维化大鼠CFB细胞内胶原的合成。
Objective To investigate the effect of moxibustion on type Ⅰ、Ⅲ and Ⅳ collagen synthesis in conlonic fibroblasts（CFB） and explore the mechanisms of its preventive and therapeutic actions on colonic fibrosis in ulcerative colitis fibrosis rats. Methods A rat model of ulcerative colitis fibrosis was made by an immunological method plus local stimulation. Rats were randomly divided into normal, model, herb-partition moxibustion, mild moxibustion and salicylazosulfapyridine（SASP） gavage groups. The herb-partitioned moxibustion and mild moxibustion groups were treated by herb-partitioned moxibustion and mild moxibustion, respectively, on points Qihai（CV 6） and Tianshu（ST 25, bilateral）. The SASP gavage group was treated by salicylazosulfapyridine gavage. Colonic fibroblasts were isolated and cultured in all the groups of rats. The effect of moxibustion on type Ⅰ、Ⅲ and Ⅳ collagen synthesis in colonic fibroblasts was investigated by western-blotting in the rats with ulcerative colitis fibrosis. Results Type Ⅰ、Ⅲ and Ⅳ collagen synthesis in colonic fibroblasts increased significantly in the rats with ulcerative colitis fibrosis. Type I and IV collagen synthesis in colonic fibroblasts was significantly inhibited in both Herb-partitioned moxibustion and mild moxibustion groups of rats. Type I collagen synthesis in colonic fibroblasts was significantly inhibited in the SASP gavage group of rats. Conclusions Moxibustion can regulate CFB collagen synthesis in ulcerative colitis fibrosis rats.
出处
《上海针灸杂志》
2009年第2期63-66,共4页
Shanghai Journal of Acupuncture and Moxibustion
基金
国家重点基础研究发展计划(2009CB522900)
关键词
间接灸
结肠炎
溃疡性
大鼠
成纤维细胞
胶原
Indirect moxibustion
Colitis, Ulcerative
Rats
Fibroblasts
Collagen
