摘要
目的探讨Amplisensor-聚合酶链反应(AmpliSensor-PCR)在结核病诊断中的价值。方法采用AmpliSensor-PCR对784例结核病患者及160例肺癌患者的标本进行检测,并与PCR(凝胶电泳后,经溴化乙锭染色)、涂片、培养等法比较。结果AmpliSensor-PCR的敏感性显著高于涂片及培养(P<0.01)。特异性较PCR法高。结论AmpliSensor-PCR可以通过标准曲线划定检出下限,并可换算出标本中原始的靶DNA值,同时具有较高的特异性和敏感性,对肺结核尤其是肺外结核的诊断有一定的临床意义。
Objective To explore the value of Amplisensor PCR method in diagnosis of tuberculosis. Method The samples from 784 patients with tuberculosis, 160 patients with lung cancer were detected by using AmpliSensor PCR, PCR, smear and culture methods, and the results were compared. Result The sensitivity of AmpliSensor PCR technique was obviously higher than that of smear and culture ( P <0.01). The specificity of AmpliSensor PCR method was higher than that of PCR method. Conclusion Amplisensor PCR method can be determined to detect the lower limit by standard curve, through which the original amount of target DNA in samples can be calculated. AmpliSensor PCR method shows higher specificity and sensitivity. It is of clinical value in diagnosis of pulmonary tuberculosis and particularly of extrapulmonary tuberculosis.
出处
《中华结核和呼吸杂志》
CAS
CSCD
北大核心
1998年第2期79-81,共3页
Chinese Journal of Tuberculosis and Respiratory Diseases
关键词
结核病
诊断
聚合酶链反应
结核杆菌
Tuberculosis Mycobacterium tuberculosis Polymerase chain reaction