摘要
背景:目前有关槟榔成分对成纤维细胞增殖的影响存在两种截然不同的结果。有学者用较低浓度的槟榔碱能促进成纤维细胞增殖,另有实验显示槟榔碱在高于15.6mg/L时对成纤维细胞的直接作用是抑制成纤维细胞生长和胶原合成。目的:进一步验证槟榔提取物对人增生性瘢痕成纤维细胞的抑制作用,为烧伤后增生性瘢痕的治疗提供实验依据。设计、时间及地点:分组对比观察,于2002年在解放军总医院烧伤研究所完成。材料:槟榔生药为北京同仁堂提供。手术中取下的2例增生性瘢痕患者组织,年龄30岁以下,病程半年～2年,患者对治疗及研究均知情同意。方法:体外培养烧伤后的人增生性瘢痕成纤维细胞,分别加入15.63,31.25,62.5,125,250,500mg/L不同剂量的槟榔提取物作用。主要观察指标:24h后观察细胞形态学,以乳酸脱氢酶为指标观察细胞毒性,以四甲基偶氮唑盐法检测细胞增殖活性。结果:加入不同剂量提取物作用24h后,培养上清液中乳酸脱氢酶与对照组无显著性差异(P>0.05);不同浓度的槟榔提取物均能改变成纤维细胞形态,抑制细胞增殖,浓度在500mg/L以下无明显的细胞毒作用。结论:槟榔提取物对人增生性瘢痕成纤维细胞具有抑制作用,并呈剂量依赖关系。
BACKGROUND： At present, thoughts regarding to effects of on hypertrophic scar fibroblasts can be divided into two parts. One is arecaline with low concentration can facilitate the fibroblasts proliferation, other is arecaline with concentrations of over 15.6 mg/L will inhibit the growth of fibroblast as well as increased collagen synthesis. OBJECTIVE： To explore the inhibitory effects of Areca catechu L. extracts on hypertrophic scar-derived fibroblasts, and to provide experimental basis for treatment of hypertrophic scar after burn. DESIGN, TIME AND SETTING： The grouping observation based on a comparison was performed at the Institute of Burns, General Hospital of Chinese PLA in 2002. MATERIALS： The crude drug of Areca catechu L. was provided by Beijing Tongrentang. The hypertrophic scar-derived tissues were obtained from 2 patients, who aged below 30, with course of disease 0.5-2 years. Informed consent was obtained from the participant. METHODS： Hypertrophic scar-derived fibroblasts were cultured in vitro with Areca catechu L. extracts with different concentrations （15.63, 31.25, 62.5, 125, 250, 500 mg/L）. MAIN OUTCOME MEASURES： The cell proliferative activity of the fibroblasts was examined by MTT method, and the cellular morphology, as well as cytotoxicity was observed after 24 hours of culture. RESULTS： At 24 hours of culture, there was no significant difference of lactic dehydrogenase in the medicine group and the blank control group （P 〉 0.05）. Areca catechu L. extracts with any concentrations could change the fibroblast morphology, and inhibit the cell proliferation. Furthermore, no significant cytotoxicity was observed in Areca catechu L. extracts less than 500 mg/L CONCLUSION： Areca catechu L. extracts exhibit inhibitory effects on human hypertrophic scar-derived fibroblasts growth with a dose-dependent manner.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第7期1247-1250,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
