摘要
运用免疫组化技术，以神经元特异性烯酸酶（NSE）和胶质纤维酸性蛋白（GFAP）作为标记物，观察大鼠实验性脑挫伤后不同存活时间中神经元和星形胶质细胞的数量变化。结果发现，伤后3h可见NSE和GFAP染色变化，且随着存活时间延长，脑皮质内挫伤处周围神经元逐步减少，而星形胶质细胞则逐渐增加，具有一定的时间规律性。为脑挫伤后早期存活时间的推断提供了新的方法。
In this study,the changes of NSE and GFAP in the forebrain of rats at various survival time(3h to 120h) after experimental brain contusion were detemined by quantitative immunohistochemistry. The results demonstrated that the variat ions of NSE and GFAP were detected at 3h, the levels of NSE were steadily decroasod and the levels of GFAP were regularly increased with increasing of the survival time. This method could be used for estimating the early survival time died from brain injury.
出处
《中国法医学杂志》
CSCD
1998年第1期7-11,共5页
Chinese Journal of Forensic Medicine
关键词
脑挫伤
免疫组化
神经元
胶质纤维
病理学
Brain contusion
Immunohistochemistry
NSE
GFAP
Survival time
