摘要
目的研究哺乳期用L-精氨酸(L-Arg)对宫内发育迟缓(IUGR)大鼠肾功能的影响及机制探讨。方法给予孕鼠10%低蛋白饲料制造IUGR仔鼠模型,并在哺乳期(21d内)给母鼠含21%蛋白饲料,添加L-Arg干预者为干预组(LT组),不添加者为IUGR组;正常对照组(NC组)全程予含21%蛋白饲料喂养。于生后1 w、3 w、2月和3月龄,留取血清标本测定肌酐(Cr)、尿素氮(BUN)和NO浓度;取后3个时间点,留取尿液,测尿肌酐(UCr)和24h尿蛋白定量。结果(1)与NC组比较,IUGR组在4个时间点血清NO的浓度均有显著降低;而LT组与正常组比较无显著差异;LT组与IUGR组相比,3 w、2月和3月龄血清NO的浓度均明显增加。(2)3w和2月龄时,三组仔鼠的24h尿蛋白定量比较无显著差异;3月龄时,IUGR组显著高于NC组和LT组。(3)三组仔鼠在4个观察点比较Cr无显著差异;2月龄时IUGR组与NC组比较,UCr有显著差异;3月龄时与NC组和LT组比较,IUGR组大鼠UCr差异有显著性。(4)与NC组和LT组比较,IUGR组CCr水平在3w、2月和3月龄均有显著减低。结论生后早期给予L-Arg干预,IUGR大鼠血清NO浓度和CCr提高、24h尿蛋白减低;IUGR仔鼠体内NO降低是CCr降低和24h蛋白尿增加的重要原因之一。
Objective To observe the influence of L-Arg supplementation during early postnatal life on renal function of rats with intrauterine growth retardation（IUGR）.Mehod Afer establishment of IUGR model with 10% protein diet,rats were divided randomly into 2 groups： IUGR group and L-Arg treated group（LT group）.Normal control group（NC group） rats were fed with 20% protein diet.The level of NO,Cr in serum were measured from 1 w to 3 months and UCr,24 h-proteinuria were measured from 3 w to 3 months.Results（1）The levels of serum NO reduced significantly in group IUGR at every stage,not significantly different between group LT and NC,but more in group LT than in group IUGR.（2）The levels of 24h-proteinuria had no significant difference among three groups at 3 w and 2 month,but was significantly higher in group IUGR than group LT and group NC.（3）The Cr levels had no significant difference at every stage among three groups.The UCr level was decreased in group IUGR than group NC at 2 month and than group NC and LT at 3 month.（4）The level of CCr in group IUGR was decreased significantly at 3 w,2 month and 3 month than group LT and group NC.Conclusion L-Arg can increase serum NO and CCr levels and decrease the level of 24h-proteinuria in rats with IUGR.It is one of the important reasons to increase 24h-proteinuria and reduce CCr level for low NO value.
出处
《营养学报》
CAS
CSCD
北大核心
2009年第1期38-41,共4页
Acta Nutrimenta Sinica
