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一种简易的检测人体血浆中沙丁胺醇浓度的HPLC法 被引量:8

A Simple and Rapid HPLC Determination of Salbutamol Concentrations in Human Plasma with Fluorescence Detection
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摘要 建立了一种简易的反相高效液相色谱方法测定沙丁胺醇血药浓度.以含磷酸二(2-乙基己基)酯的氯仿液进行提取,0.1mol/L盐酸反提,内标为丁酚胺.固定相为SpherisorbC18;流动相为:0.03mol/L磷酸二氢铵(用磷酸调至pH2.6)—甲醇—乙腈(92.2∶3.4∶4.4),流速1.5mL/min.荧光检测器的激发波长为225nm,荧光波长为310nm.在血浆浓度0.5~50ng/mL范围内线性良好.回收率为78.3%~83.6%.日内相对标准偏差为4.5%~6.8%,日间相对标准偏差为7.2%~9.5%.应用本方法测定了8名志愿者口服沙丁胺醇片后的血药浓度. A simple and rapid HPLC method is presented for the determination of salbutamol in human plasma.Plasma samples were extracted with chloroform containing di(2ethylhexyl)phosphate.Samples were back extracted into a hydrochloric acid solution.This method involved a C18 column as stationary phase and 003mol/L (NH4)H2PO4(pH26,adjusted by H3PO4)-methanol-acetonitrile(922:34:44)as mobile phase.A fluorescence detector was used for this assay.Bamethane was used as the internal standard.The carlibration curve was linear between 05 and 50 ng/mL with a regression coefficient of 0999. The recoveries of salbutamol samples were 783%~836% for plasma. Inter-day and intra-day RSD were 45%~68% and 72%~95% respectively. This method has been used in the bioavailability study of salbutamol tablets (Ventolin, Glaxo) given to eight healthy volunteers.
作者 陈孝 赵香兰
出处 《药物分析杂志》 CAS CSCD 北大核心 1998年第2期98-98,共1页 Chinese Journal of Pharmaceutical Analysis
关键词 沙本胺醇 高效液相色谱 药代动力学 血药浓度 salbutamol,HPLC,pharmacokinetics
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