摘要
以茄子栽培种(Solanum melongena)自交系‘E-35’、‘E-38’、‘E-8’、‘E-30’与野生种水茄(Solanum torvum)为亲本进行种间杂交,获得了E-35×Solanum torvum的种间杂种。对F1杂种进行形态学、RAPD、GISH分析以及抗病性鉴定。结果表明:种间F1多数形态性状居中,花粉具有较高的活力,具有可稔性。RAPD以及GISH分析表明,F1植株不仅包含双亲带型,而且产生新的特征带,含有栽培茄与野生茄染色体,染色体数为2n=24,表明所得的F1杂种为真正的种间杂种。杂种经过人工接种及田间发病鉴定,表现高抗青枯病,其抗青枯病性状可能由两对显性基因控制。该种质可以作为茄子抗病育种的一个新抗源。
In order to obtain the new disease-resistance resource in eggplant, inter-specific hybridization was made with pollination between Solanum melongena and Solanum torvum inbreeding lines, ＇ E-8 ＇ , ＇ E- 30＇ , ＇E-35＇ , ＇E-38＇. Only the inter-specific hybrid of ＇E-35＇ × Solanum torvum was obtained. The hybrid were identified by morphology, RAPD and GISH methods, the disease-resistance of the hybrid was tested by inoculation. The results showed that the morphological characteristic of F1 was intermediate to that of its parent, the pollen viability of F1 was high, its seed was fertile. Not only its parent characteristic bands, but also some new characteristic bands of hybrid were observed in F1 plant, and the chromosome of both parent （Solanum melongena ＇E-35＇ and Solanum torvum） were determined in F1, its number of chromosome was about 2n = 24, those showed that the F1 was a real inter-specific hybrid. The hybrid showed high bacterial wilt resistance by inoculated to race 1 of bacterial wilt and in the field, the resistance to bacterial wilt may be controlled by two dominance genes. So the inter-specific hybrid can be used as a new resource with resistance to bacterial wilt for eggplant breeding.
出处
《园艺学报》
CAS
CSCD
北大核心
2009年第2期209-214,共6页
Acta Horticulturae Sinica
基金
广东省科技攻关项目(2007A020300009-4)
广州市科技局攻关项目(2005220071)
关键词
茄子
野生茄
种间杂种
杂种鉴定
eggplant
Solanum rnelongena
Solanum torvum
inter-specific hybrid
hybrid identification
