摘要
目的在体外培养骨膜细胞成功的基础上，进一步探讨骨膜细胞移植对缺血性坏死的股骨头的修复作用。方法在无菌条件下切取新生犬的胫骨骨膜，进行骨膜细胞培养。将８只成年犬分成实验组和对照组，建立股骨头缺血性坏死模型。２周后，将实验组培养的骨膜细胞移植到缺血性坏死的股骨头内，对照组不做骨膜细胞移植。６周后取出股骨头进行组织病理学检查。结果实验组缺血坏死的股骨头内有大量新骨形成，新生骨质呈小梁状排列，小梁表面常有单层的成骨细胞覆盖，提示新骨形成活跃；小梁间的纤维肉芽组织内血管丰富。而对照组的股骨头内骨小梁萎缩变细，骨质坏死；小梁间被纤维肉芽组织充填，血管稀少。
Objective To investigate the effect of periosteal cell transplantation on the repair of avascular necrosis of femoral head (ANFH). on the basis of successful culture of periosteal cells in vitro. Methods The tibial periostea of new born dog were excised and cultured under aseptic conditions. Eight adult dogs were used and divided into 2 groups: experimental group and control group. The animal model of ANFH was made by transferring the freed femoral head into the muscle under the ilium on one side in each dog. Two weeks after the establishment of ANFH, the cultured periosteal cells were collected and transplanted into the necrotic femoral heads in the experimental group, and the femoral heads without periosteal cell transplantation served as the control. Six weeks after cell transplantation, the femoral heads were taken out and histological examination was made.Results There was active bone formation in the experimental group. The new bone was arranged into bone trabeculae, most of which were covered by a monolayer of osteoblasts. Between bone trabeculae were fibrous tissue rich in blood vessels. In the conrol group, the bone trabeculae withered and remained necrotic. There were fibrous tissues with few blood vessels between bone trabeculae.Conclusion Periosteal cell transplantation can improve the repair of ANFH.
出处
《中华医学杂志》
CAS
CSCD
北大核心
1998年第1期52-55,共4页
National Medical Journal of China
