摘要
目的建立盆炎灵合剂的质量标准。方法采用TLC法对盆炎灵合剂中当归、延胡索药材进行定性鉴别 用HPLC法对芍药苷进行定量研究。色谱柱为Agilent C18（250 mm×4.6 mm,5μm）。流动相为甲醇-0.1%磷酸溶液（25∶75）,流速为1.0 mL.min-1,检测波长为230 nm,柱温为35℃。结果在TLC色谱中均能检出当归、延胡索,芍药苷在0.015~0.15 mg.mL-1与峰面积呈良好的线性关系（r=0.999 9）,平均回收率为98.66%,RSD为0.94%（n=9）。结论该方法操作简便,结果准确,灵敏度高,重现性好,适用于盆炎灵合剂的质量控制。
Objective To establish the quality standard of penyanling mixture. Methods Angelica sinensis and rhizoma corydalis in penyanling mixture were indentified by TLC. Peoniflorin was determined by HPLC. The HPLC was conducted on an AgiLent C18 column （250 min×4.6 mm, id 5 μm） with anol-0. 1% phosphoric acid （25 ： 75） solution, which was used as mobile phase with flow rate of 1.0 mL · min-1. The detective wavelength was 230 nm and column temperature 35 ℃. Results Angelica sinensis and rhizoma corydalis could be detected by TLC. The area under the peak of chromatography （r=0. 999 9） was very well correlated with the concentration of peoniflorin at 0. 015- 0. 15 mg · mL-1. The average recovery was 98.66%, RSD=0. 94% （n=9）. Conclusion The method is simple and accurate. The experiment is reproducible. The method can be used for the quality control of penyanling mixture.
出处
《中南药学》
CAS
2009年第2期122-124,共3页
Central South Pharmacy
