摘要
为开展伤寒六经病证的动物模型研究,采用先灌服大剂量寒凉药7天,再结扎左冠状动脉前降支,逐渐缩窄升主动脉口径的方法,实验7～9天后,动物出现倦卧,活动减少,欲睡,毛发枯燥,嘴及耳部发绀,足部水肿,四肢与腹部皮肤温差较假手术组扩大(P<0.001)。处死动物后发现结扎处心肌坏死,其它处心肌肥厚,肝脏出现瘀斑,心、左心室、心重/体重指数、肝、肝重/体重指数均较假手术组为高(P<O.05)。与假手术组对比左室内压明显上升(P<O.01),其左室内压最大上升速率也明显增加(P<0.05),成功地建立了充血性心力衰竭少阴病阳虚水停证兔动物模型。
By first infusing large dose cold nature drugs, followed by ligating the anterior descending branch of left coronary artery and gradually narrowing down their aortic calibre, animal models were made 7-9 days after the operation, manifesting lassitude, drowsy, withered hairs, cyanotic lips and auricles, dropsy of feet. The surface temperature difference of extremities and abdomen was expanded than that of the pseudooperated animals (P<0. 001). It was found in the killed animals that the myocardium was necrotic at the ligated site with hypertrophy of other heart muscles, ecchymosis of liver tissue, the indices of heart body weight, liver and liver weight/body weight/were markedly elevated as compared with those of pseudo operated animals (P<0. 05). The left intraventricular pressure was also higher (P<0. 01) the maximal increase velocity of its left ventricular pressure was also greatly increased (P<0. 05). Thus, the animal model was succesfully established.
出处
《中医杂志》
CSCD
北大核心
1998年第3期177-179,共3页
Journal of Traditional Chinese Medicine
关键词
充血性心力衰竭
疾病模型
heart failure, congestive, Shaoyin disease disease model, animal
