摘要
以栽培黄芩(Scutellaria baicalensisGeorgi)内生真菌菌株SBO23为试验菌株,以大肠杆菌(Escherichia coli),枯草芽孢杆菌(Bacillus subtilis),深红酵母(Rhodotorula rubra)为指示菌,研究了不同发酵条件对菌株SBO23抑菌活性的影响.结果表明,抑菌活性最佳的碳源为葡萄糖,氮源为胰蛋白胨,需氧量为120 r/m转速条件下,装液量70 mL/250mL,起始pH8.0,温度24℃,发酵培养8 d时,抑菌效果最好.
A strain of endophytic fungus SBO23, which was isolated from cultivated ScuteUaria baicalensis Georgi, was used in this experiment. The inhibitory activity test to three species of microbe （ Bacillus subtilis, Escherichia coli, Rhodotorula rubra ） were conducted to research. The objective was to explored the effects of differmt fermentation conditions on anti- microbe activity of endophytic fungus SBO23. The optimal condition for SBO23 possessed high anti-microbe activity were as following： sucrose and starch were suitable C source, Tryptone was the suitable N source, a 120 r/min shake revolutions with 70 mL medium in 250 mL flask, culture temperature 34 ℃, initial pH8.0 and culture time 8 days, the diameter of inhibition zone was the longest.
出处
《首都师范大学学报（自然科学版）》
2008年第4期42-45,58,共5页
Journal of Capital Normal University：Natural Science Edition
关键词
黄芩
内生真菌
发酵条件
抑菌活性
Scutellaria baicaleusis Georgi, endophytic fungi, fermentation condition, inhibitory activity
