摘要
目的探讨以调理脾胃气机法组成的中药复方解郁1号对SS调衡HPA轴功能的干预机制。方法60只SD大鼠随机分为正常对照组、模型对照组、阿米替林组及解郁1号组,应用孤养加慢性轻度不可预见性应激方法复制大鼠抑郁症模型,采用放射免疫法测定血浆促肾上腺皮质激素释放激素(CRH)、生长抑素(SS)、皮质醇(CORT)的含量,分析SS、CRH、CORT之间的相关性,初步探讨中药复方解郁1号对SS调衡HPA轴功能的影响,以期进一步阐明本方的抗抑郁机制。结果SS与CRH之间的相关系数为r=-0.5094,P=0.1972,t=1.4501,结果表明SS与CRH呈显著负相关;SS与CORT之间的相关系数为r=0.010,P=0.9613,t=0.0244,结果表明SS与CORT之间无明显相关性。结论SS可能通过抑制CRH的释放,达到对HPA轴的调衡作用。而解郁1号则可能通过对SS调衡HPA轴功能的干预,发挥其抗抑郁效应。
Objective To investigate the affecting mechanism of Jie Yu No. 1 Decoction（JY） ,which was a complex prescription with the function of adjusting spleen and stomach, on the HPA - axis function of depression model rats. Methods 60 SD rats were randomly divided into the normal, the model, the Amitriptyline and the JY decoction groups,with 15 each. Isolated -living condition and chronic mild unpredictable stress were applied suceessively to set up DEP rat model. Radiative immunotherapy was used to measure the content of hormone（CRH） , somatostatin （SS） and Cortisol （CORT） in the blood plasma. The correlativity was Analyzed between SS, CRH and CORT to preliminary discuss the affecting mechanism of JY on the function of SS adjusting HPA - axis, and to clarify the antidepressant mechanism of the JY. Results The correlation coefficient between SS and CRH was r = - 0. 5094,P = 0. 1972, t = 1. 4501. The result indicated that SS and CRH had the remarkable negatively correlation. The correlation coefficient between SS and CORT was r = 0. 010,P = 0. 9613,t = 0. 0244. The result indicated that SS and CORT had no remarkable correlation. Conclusion The antidepressant mechanism of JY may be as that SS reduces CRH contents and reaches the adjustment of HPA - axis function in order to play its antidepressant effect.
出处
《医学研究杂志》
2009年第3期38-39,共2页
Journal of Medical Research
基金
国家自然科学基金项目(30660231)
