摘要
采用美国ABI公司牛亲子鉴定试剂盒(Bovine Paternity PCR Typing Kit,包括11个常染色体)和3个自选的Y染色体微卫星座位,检测我国部分种公牛站肉用种公牛14个微卫星座位的多态性分布,评估其遗传多样性,并探讨其用于个体识别与亲缘鉴定的可行性。结果表明:种公牛在14个微卫星座位中遗传多样性均较高,其中MCM158座位的平均多态信息含量最高达到0.888,ETH10座位的最低,为0.482。单个座位的个体识别能力在0.715～0.968之间,累积个体识别能力为99.99%,累计非父排除率达到99.99%,表明采用的14个位点适用于个体识别和亲缘鉴定。
The polymorphism distributions of 14 microsatellite loci were detected using the Bovine Paternity PCR Typing Kit （includingl 1 X-STR） and 3 selected Y-STR microsatellite DNA markers. The genetic diversity were evaluated, and the feasibility of the application to individual identification and paternity testing were discussed. The results showed that all the 14 microsatellite loci had genetic polymorphisms in bulls, and the polymorphism information content （PIC） in loci MCM158 was the biggest （0.888）, while the ETHIO was the lowest （0.482）. Power of discrimination （DP） value of the 14 STR loci ranged from 0.715 to 0.968. The Cumulate DP （CDP） was 99.99%, and the Cumulate PE （CPE） also reached 99.99%. These results indicate that the 14 microsatellites can be applied to the individual identification.
出处
《遗传》
CAS
CSCD
北大核心
2009年第3期285-289,共5页
Hereditas（Beijing)
基金
农业部畜禽牧草品种资源保护项目(编号:农财发[2007]43号)资助
关键词
种公牛
微卫星
个体识别
亲缘鉴定
bull
microsatellite
individual identification
paternity testing
