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吸入一氧化氮对脂多糖所致大鼠急性肺损伤环氧合酶-2及TOLL样受体4表达的影响 被引量：3

The effect of nitric oxide inhalation on the expression of COX-2 and toll-like receptor 4 in rat acute lung injury induced by fipopolysaccharide
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摘要 目的建立脂多糖致大鼠急性肺损伤的模型，观察一氧化氮对大鼠气道环氧合酶-2（COX-2）和Toll样受体4（TLR4）的分布及表达的影响。方法健康雄性sD大鼠24只，按随机数字表法分为正常对照组、单用脂多糖（脂多糖组）、脂多糖加NO20×10^-6mg／L（低浓度NO组），脂多糖加NO100×10^-6mg／L（高浓度NO组）。气管内滴注脂多糖（5mg／kg）建立大鼠急性肺损伤模型。观察6h后的肺水肿程度、免疫组织化学（sP染色）和实时荧光定量PCR检测肺组织中COX-2和TLR4的表达，以及分别吸入20×10^-6、100×10^-6mg／L浓度NO后的影响。所得数据采用单因素方差分析进行统计学分析，多个样本均数之间的两两比较采用LSD—t检验。结果COX-2和TLR4在对照组大鼠气道内有广泛的分布和表达。脂多糖组大鼠肺水肿程度明显高于对照组，其主支气管和肺内细支气管上皮细胞内COX-2（6．5±2．8）及TLR4（44．9±11．3）表达高于对照组（分别为2．8±0．8、2．1±0．7），差异有统计学意义（t值分别为3．003、10．480，均P〈0．01）。低浓度NO组肺水肿程度明显减轻，其COX-2表达量（5．0±2．0）低于脂多糖组，但差异无统计学意义（t=1．227，P〉0．05）。而低浓度NO组TLR4（16．2±3．8）的表达量与脂多糖组（44．9±11．3）比较差异具有统计学意义（t=7．030，P〈0．001）。结论COX-2和TLR4在大鼠气道内广泛分布，脂多糖刺激可使COX-2和TLR4的表达增强，吸入适当浓度NO可降低由脂多糖引起的COX-2和TLR4表达的增高。 Objective To study the effect of nitric oxide （NO） on the expression of cyclo-oxgenase 2 （COX-2） and toll-like receptor 4 （TLR4） in a rat model of acute lung injury （ALI） induced by lipopolysaccharide （LPS）. Methods Twenty-four Sprague-Dawley rats were randomly divided into 4 groups. Group N： normal control group; Group L： ALI model by LPS intratracheal instillation; Group Za： ALI model ＋ inhaled NO 20 × 10^-6 mg/L; Group Zb： ALI model ＋ inhaled NO 100 × 10^-6 mg/L. Lung morphology was studied and COX-2 was detected by immunohistochemistry（IHC） while TLR4 by fluorescent quantitative PCR（FQ-PCR）. Results Immunohistochemistry and FQ-PCR showed that COX-2（2. 8 ± 0. 8 ） and TLR4 （ 2. 1±0. 7 ） were detected in the respiratory tract of the normal control rats. In Group L, the expression of COX-2 （ 6. 5 ± 2. 8 ） and TLR4 （ 44. 9± 11.3 ） was increased in the main bronchus and bronchioles, compared to the normal controls （ t = 3. 003, 10. 480, both P 〈 0. 01 ）. In Group Zb, the expression of COX-2 （ 5.0 ± 2. 0 ） and TLR4 （ 16. 2 ± 3.8 ） were decreased as compared to Group L, but only the level of TLR4 showed statistical difference （ t = 7. 030, P 〈 0. 001 ）. Conclusions COX-2 and TLR4 distributed widely in the respiratory tract of the rats. LPS increased the expression of COX-2 and TLR4. Low dose of nitric oxide （20 × 10^-6 mg/L） inhalation reduced the bronchiolar expression of COX-2 and TLR4 induced by LPS.
作者 吴妍雯 白明 张建初 金阳 张蕾 包滨
机构地区 华中科技大学同济医学院附属协和医院呼吸科
出处 《中华结核和呼吸杂志》 CAS CSCD 北大核心 2009年第2期111-114,共4页 Chinese Journal of Tuberculosis and Respiratory Diseases
基金 湖北省自然科学基金资助项目（2006ABA145）
关键词 呼吸窘迫综合征 成人 环氧化酶2 TOLL样受体4 一氧化氮 脂多糖类 Respiratory distress syndrome, adult Cyclooxygenase 2 Toll-like receptor 4 Nitric oxide Lipopolysaccharides
分类号 R686 [医药卫生—骨科学]
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共引文献53

同被引文献33

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中华结核和呼吸杂志
2009年 第2期

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