摘要
目的:观察姜黄素对溶血磷脂酰胆碱(LPC)诱导内皮细胞炎症因子产生的影响,探讨姜黄素抗动脉粥样硬化(AS)的作用机制。方法:体外培养的人脐静脉内皮细胞(HUVECs),待细胞生长到融合状态时加入不同浓度(25、50、100 mg/L)的姜黄素预处理30 min,然后加入LPC(5 mg/L)作用24 h,用酶联免疫吸附法(ELISA)检测上清液中细胞间黏附分子-1(ICAM-1)、单核细胞趋化蛋白-1(MCP-1)、IL-6和TNF-α的含量;实时荧光定量PCR(real-time PCR)检测ICAM-1、MCP-1、IL-6和TNF-α mRNA的表达;蛋白印迹法(Western blot)和免疫细胞化学染色法检测核因子-κBp65(NF-κBp65)蛋白的表达。结果:LPC能明显增加HUVECs ICAM-1、MCP-1、IL-6、TNF-αmRNA和NF-κBp65蛋白的表达以及上清液中ICAM-1、MCP-1、IL-6、TNF-α的含量,而预先应用不同浓度的姜黄素干预后,上述效应明显减弱,并且具有剂量依赖性。结论:姜黄素抑制炎症因子的产生可能与其抑制NF-κB的活性有关。
AIM： To observe the effects of curcumine on the production of inflammatory factors induced by lysophosphatidylcholine （LPC） and investigate the anti-atherosclerosis mechanism of curcumine. METHODS： The human umbilical vein endothelial cells （HUVECs） were treated with LPC （5 mg/L） for 24 h following pretreatment with various concentrations of cureumine（25,50,100 mg/L） for 30 min. Intercellular adhesion molecule-1 （ ICAM-1 ）, monocyte chemoattractant protein-1 （MCP-1）, IL-6 and TNF-α were determinded by enzyme-linked immunosorbent assay （ELISA） and real-time quantitative reverse transcription-polymerase chain reaction （real-time RT-PCR）, the protein expression of nuclearfactor kappa Bp65 （NF-κBp65） was assayed by western blot analysis and immunohistochemieal method. RESULTS： The levels and mRNA expression of ICAM-I, MCP-1, IL-6 and TNF-α, and the NF-κBp65 activity were significantly increased by LPC. The increased production of inflammatory factors and NF-κBp65 activity were markedly inhibited by different concentrations of cureumine in a dose-dependent manner. CONCLUSION： Curcumine inhibited the production of inflammatory factors might be related with depressing the activation of NF-κB.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2009年第1期52-56,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
关键词
姜黄素
溶血磷脂酰胆碱
内皮细胞
动脉粥样硬化
炎症因子
curcumine
lysophosphatidylcholine
endothelial cells
atherosclerosis
inflammatory factor
