摘要
在摇瓶液体培养条件下,研究了不同培养条件对冬虫夏草液体种子细胞生长的影响。实验结果表明,冬虫夏草液体种子细胞生长的最宜条件为:培养温度24℃,转速150r/min,装液量30%(75mL/250mL),初始pH6.0。在此条件下,经摇瓶培养,获得最大菌体干重为11.218g/L。
Cordyceps sinensi was inoculated and cultivated under submerged cultivation conditions in the shake flask. The result indicates that the optimum cultivation conditions for Cordyceps sinensis cell growth were as follows： cultivation temperature of 24 ℃, rotation speed of 150 r/min, the medium volume in the flask of 30% （75mL/250 mL）, and initial pH of 6.0. Under those conditions, the highest dry weight of the thalli （11.22 g/L） is achieved.
出处
《现代食品科技》
EI
CAS
2009年第2期188-190,共3页
Modern Food Science and Technology
关键词
冬虫夏草
培养条件
温度
转速
装液量
初始pH
Corclyceps sinensis
culture conditions
temperature
rotation speed
the medium volume in the flask
initial pH
