摘要
应用实时荧光PCR技术对转基因大米进行了定性和定量检测研究。本研究以转基因Bt63大米为材料,采用TaqMan探针技术,对大米中的内源基因蔗糖磷酸合酶SPS和转基因水稻中普遍存在的外源基因CaMV35S启动子、NOS终止子以及苏云金芽孢杆菌(Bacillus thuringiensis,简写为Bt)杀虫晶体蛋白基因Cry1Ac进行了实时荧光PCR研究,并对外源基因Cry1Ac进行了定量检测和敏感性分析。该实时荧光PCR方法检测结果和常规PCR结果一致,同时不用进行凝胶电泳,更为快速、简便,降低了污染机会,可用于转Bt基因大米的定性和定量检测。
Real-time fluorescence PCR was applied to qualitative and quantitative analysis of genomic modified Bt63 rice. The specific primers and TaqMan fluorescence probes were designed to test the endogenous SPS gene and exogenous genes of promoter CaMV35S, terminator of NOS and CrylAc gene. Quantitative test anti sensitivity analysis was also performed according to CrylAc gene. The results showed that the real-time fluorescent PCR method was more convenient and quickly, less contamination than common PCR, which was suitable for qualitative and quantitative analysis oftransgenic Bt63 rice was established.
出处
《现代食品科技》
EI
CAS
2009年第2期211-216,220,共7页
Modern Food Science and Technology
