摘要
【目的】鉴定影响籽粒大小相关性状的QTL,并估计QTL的表型效应;分析不同环境下QTL的稳定性。【方法】以冬小麦小粒地方品种和尚麦为母本,大粒育成品种豫8679为父本及其F7:8重组自交系的142个株系为试验材料,分析籽粒长度、宽度、厚度、体积及千粒重在北京(2006、2007)、合肥(2007)和成都(2007)4个不同环境下的性状表现,并利用已构建的含有170个SSR标记和2个EST标记的遗传图谱,对这5个性状进行QTL定位分析。【结果】4个环境下共检测到93个影响籽粒长度、宽度、厚度、体积及千粒重的QTL,这些QTL分布在除1D和6A之外的所有小麦染色体上。在检测到的QTL中,与籽粒长度、宽度、厚度、体积和千粒重相关的QTL分别为17、16、18、21和21个。另外,本研究还在1A、1B、2A、2D、3A、3B、5A、5B、5D、6A、6D、7B和7D染色体上共发现了18个QTL富集区。【结论】获得93个影响小麦籽粒大小相关性状的QTL,这些QTL可作为利用分子标记辅助育种途径进行小麦遗传改良的依据。
[Objective] QTL associated with grain size and related traits was identified and the phenotypic effects of them were estimated. The stability of QTL across different environments were detected. [Method ] Using a set of 142 recombinant inbred lines （RILs） derived from Chinese winter wheat varieties Yu8679 and Heshangmai, five agronomic traits of grain length （GL）, grain width （GW）, grain thickness （GT）, grain volume （GV） and 1000-grain weight （TGW） were evaluated in four different ecological environments of Beijing （2006 ＆2007）, Hefei （2007） and Chengdu （2007）, respectively. Based on 142 RILs, a genetic map comprising 170 SSR markers and two EST markers （Tx23-24 and Tx37-38） was generated. According to the genetic map and phenotypic data, quantitative trait loci （QTL） were located for these agronomic traits using the composite interval mapping method. [Result] A total of 93 QTL involving all wheat chromosomes except 1D and 6A were identified for these five traits over four environments. Among them, 17, 16, 18, 21 and 21 QTL for GL, GW, GT, GV and TGWwere identified, respectively. In addition, 18 genomic regions with plenty of QTL were detected on 1A, 1B, 2A, 2D, 3A, 3B, 5A, 5B, 5D, 6A, 6D, 7B and 7D in this study. [Conclusion] In the present study, 93 QTL for grain size and related traits were detected, which will provide useful information for molecular assistant selection （MAS） in wheat genetic improvement.
出处
《中国农业科学》
CAS
CSCD
北大核心
2009年第2期398-407,共10页
Scientia Agricultura Sinica
基金
农业部农业结构调整重大技术研究专项(06-02-03B)
关键词
普通小麦
QTL
重组自交系
粒长
粒宽
粒厚
籽粒体积
千粒重
common wheat （Triticum aestivum L.）
QTL
RILs
grain length
grain width
grain thickness
grain volume
thousand grain weight
