摘要
目的针对医学院校生物化学课所开设的脱氧核糖核酸DNA的提取与成分鉴定原实验方法中,学生实验结果不太理想而进行实验改进。方法采用不同氯化钠浓度比差别法来抽提DNA蛋白;用SDS处理后再用氯仿-异戊醇抽提除去蛋白质,加入乙醇脱水析出DNA。用化学显色法作DNA成分鉴定。结果实验经过改进后效果理想,实验结果观察现象明显,学生实验成功率提高,实验成本降低。
Objectivet In this paper, the experiment of biochemistry course of ＂DNA Extraction and composition identification＂ is improved. Methods Different concentrations of sodium chloride are adopted to extract DNA protein; deal with SDS followed by chloroformisoamyl alcohol extraction to remove protein, precipitated by adding ethanol dehydration DNA; Colored by chemical method for identification of DNA components. Results After improvement, experiments are satisfactory, the experimental results are obvious which can improve the success rate of students in the experiment and lower the cost.
出处
《佛山科学技术学院学报（自然科学版）》
CAS
2009年第1期86-88,共3页
Journal of Foshan University(Natural Science Edition)
关键词
DNA提取
鉴定
实验
改进
DNA extraction
identification
experiment
improvement
