摘要
目的研究过度训练致肾组织黏附分子表达的改变及东莨菪碱的保护作用。方法采用大鼠游泳至力竭建立过度训练致急性肾损伤模型。将大鼠随机分为7组，即安静对照组、力竭运动后即刻组、力竭运动后6h组、力竭运动后12h组、力竭运动后24h组、山莨菪碱6h组、山莨菪碱24h组，用全自动生化仪检测各组大鼠血BUN、Scr、CK及尿r-GT的改变；光镜观察各组肾组织结构的改变；免疫组织化学方法检测各组大鼠肾组织ICAM-1、E-Cadherin表达，运用免疫组化分析软件对所选视野内阳性信号进行图像分析。结果力竭后ePN组大鼠血BUN、CK升高（P〈0．05），力竭6h后升高明显（P〈0．01），力竭12h后均有所下降，此时尿r-GT明显升高（P〈0．01），力竭24h后血BUN、CK均恢复到正常，尿r-GT继续升高（P〈0．01）。力竭运动各组Scr水平均较正常对照组略有升高，但无明显差异（P〉0．05）。光镜下力竭后即刻组大鼠肾组织结构仅见部分肾小球囊及肾小管扩张，在皮髓质交界处及髓质小血管内可见大量红细胞堆积，力竭12h大鼠可见肾小管上皮细胞刷状缘不规整，部分脱落，肾小管管腔内可见颗粒管型和透明管型。应用东莨菪碱后，力竭后6h及24h血BUN、Cr、CK及尿r-GT均明显降低（P〈0．05），肾组织结构改善。力竭运动组肾脏皮质区及髓质区ICAM-1表达随力竭后恢复时间延长呈进行性增高，E-Cadherin表达则呈进行性减弱；各力竭组与正常对照组之间均有明显差异（P〈0．01）；东莨菪碱可明显降低力竭运动后ICAM-1表达，与同时段力竭运动组相比有明显差异（P〈0．01），与正常对照组之间则无明显差异（P〉0．05）。东莨菪碱可增强E-Cadhefin的表达，与同时段力竭运动组有明显差异（P〈0．01），与正常对照组无明显差异（P〉0．05）。结论过度训练可引起急性肾损伤，肾组织黏附分子表达的变化是过度训练致急性肾损伤的重要发病机制，东莨菪碱能减轻过度运动造成的肾组织损伤，下调ICAM-1表达，增强钙黏附分子及其连接素的表达，维持了细胞正常黏附联结的存在，改善了过度训练时异常的血流动力学状态，发挥了明显的肾脏保护作用。
Objectives To study expressions of adhesion molecules in rats with overtraining - induced a- cute kidney injury and the protective efect of scoplamine on the damage. Methods The animal model of renal damage induced by overtraining was developed by exhaustive swimming. The changes of renal function were meas- ured. The renal tissue structure was observed by microscopy. Immunohistothemical technigue was used to estimate the expression of ICAM-1,E- cadherin ,in renal tissue and the. Results Were analyzed semi - quantitatively with the pathological image analysis system. The serum biochemical parameters, such as BUN and CK increased immedi- ately after exhausted rat. It was more prominent at 6 hour and recovered to the normal at 24 hour. r - GT in the urine was increased at 12 and 24 hours after exhausted rat. The morphologic changes were slight under light microscopy in HE staining. After using of scoplamine,compared with that of control rats, renal, function was improved,no morphologic changes can be found. Expressions of ICAM - 1 were increased gradually in the glomeruli and tubules of kidneys in S group rats following the time continued after exhausting exercise. But expressions of E - cadherin and β- Catenin were contrary to ICAM -1. Their expressions were reduced significantly in S24 group than N group（ p〈0.01）.After treated with scopolamine , those levels of adhesion molecules were not significant difference from those of N group rats（ p 〈 0.05 ）. Conclusions Renal damage could be induced by exhaustive swimming, These changes of adhesion molecules are the important pathogenesis of overtraining - induced acute kidney injury, scopolamine had the protective efect on the damage.
出处
《国际泌尿系统杂志》
2009年第2期150-154,共5页
International Journal of Urology and Nephrology
关键词
肾
细胞粘着分子
东莨菪碱
大鼠
Kidney
Cell Adhesion Molecules
Scopolamine
Rats
