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PIWIL4在人卵巢癌中的表达及其功能研究 被引量：8

The Expression and Functional Research of PIWIL4 in Human Ovarian Cancer
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摘要 采用半定量RT-PCR方法检测人卵巢透明细胞癌ES-2细胞中PIWIL1、PIWIL2、PIWIL3、PIWIL4 mRNA表达水平,以及PIWIL4在卵巢癌组织和癌旁正常组织中的表达情况.设计并化学合成针对PIWIL4的siRNA,用脂质体转染法将其转入ES-2细胞内,通过MTT和克隆形成实验,观察PIWIL4-siRNA对ES-2细胞生长活性和增殖能力的影响.半定量RT-PCR实验结果发现,ES-2细胞中,PIWIL4相对PIWIL1、PIWIL2、PIWIL3,其表达水平最高(P<0.05),而且PIWIL4在卵巢癌组织中的表达明显高于癌旁正常组织(P<0.01).MTT实验和克隆形成实验结果显示,转染PIWIL4-siRNA的ES-2细胞生长活性和克隆形成率明显低于对照组(P<0.05).由此得出结论:PIWIL4在卵巢癌细胞系ES-2细胞表达较高,且它在卵巢癌组织中的表达明显上调,同时PIWIL4-siRNA可有效抑制ES-2细胞的生长活性和增殖能力,提示PIWIL4可能与卵巢癌发生、发展相关. To explore the expressional information of PIWIL4 in human ovarian cancer, semi-quantitative reverse transcription polymerase chain reaction （semi-qRT-PCR） was used to detect the mRNA expression level of PIW/L1, P/W/L2, PIWIL3 and PIWIL4 in human ovarian clear cell carcinoma ES-2 cells. Meanwhile, in human ovarian cancer and adjacent normal tissues, the mRNA expression of PIWIL4 was determined. Then, PIWIL4-siRNA, which was designed to target PIWIL4 and chemical synthesized, was transfected into ES-2 cells with lipofectamine. MTT assay and clone formation assay were used to investigate the effects of PIWIIA-siRNA on the cell growth activity and proliferation capacity of ES-2 cells. Experimental results showed that the mRNA expression level of PIWIL4 was the highest compared to PIWIL1, PIWIL2 and PIWIL3 in ES-2 cells （P 〈 0.05）, and the expression of PIWIL4 mRNA in ovarian cancer tissues was higher than that in adjacent normal tissues （P 〈 0.01）. MTT and clone formation analyses demonstrated that the cell growth activity and clone formation ratio of PIWIL4-siRNA transfected ES-2 cells were markedly decreased （P 〈 0.05）. In conclusion, the expression of PIWlL4 mRNA was highest in ovarian cancer cell line ES-2 cells and was significantly increased in ovarian cancer tissues. Moreover, PIWIIA-siRNA can efficiently inhibit the cell activity and proliferation ability of ES-2 cells. In a word, PIWIL4 may be related to the pathogenesis of human ovarian cancer.
作者 郭利敏 刘民 李欣 汤华
机构地区 天津医科大学基础医学院
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2009年第3期353-357,共5页 Progress In Biochemistry and Biophysics
基金 天津市科技发展计划重点项目(033182911)~~
关键词 卵巢癌 PIWIL4 增殖 RNA干扰 SIRNA ovarian cancer, PIWIL4, proliferation, RNA interference, siRNA
分类号 Q527 [生物学—生物化学] R737.31 [医药卫生—肿瘤]
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参考文献13

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共引文献14

同被引文献103

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  • 2胡晓慧,蒋菊香,董宁征,沈飞,阮长耿.人基质金属蛋白酶-2纤维连接蛋白样片段的原核表达及单抗制备[J].苏州大学学报（医学版）,2005,25(1):27-30. 被引量：1
  • 3胡晓慧,沈飞,董宁征,李佩霞,阮长耿.抗人基质金属蛋白酶-2的单抗制备及功能研究[J].中国病理生理杂志,2005,21(5):876-881. 被引量：4
  • 4张兰,张德志.天胡荽的研究进展[J].现代食品与药品杂志,2007,17(1):15-17. 被引量：29
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引证文献8

二级引证文献7

生物化学与生物物理进展
2009年 第3期

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