摘要
目的探讨IL-1β对人椎间盘髓核细胞表达基质金属蛋白酶MMP-1、2、9、13的作用。方法分离人椎间盘髓核细胞进行单层培养并利用甲苯胺蓝、番红O染色和Ⅱ型胶原免疫细胞化学染色进行鉴定,而后分别用10ng/ml和50ng/ml重组人IL-1β刺激体外培养的髓核细胞,RT-PCR检测基质金属蛋白酶-1、2、13的表达,定量PCR检测基质金属蛋白酶-9的表达。结果10ng/ml和50ng/ml重组人IL-1β均可促进髓核细胞基质金属蛋白酶-1、2、9、13的表达(P<0.05);基质金属蛋白酶-9、13表达随IL-1β浓度升高而升高(P<0.05)。结论IL-1β可以促进人椎间盘髓核细胞表达基质金属蛋白酶-1、2、9、13,其加速了椎间盘基质分解的作用亦可能通过上述细胞因子的介导。
Objective To evaluate the effect of IL-1β on the expression of MMP-1,2,9,13 in human nucleus pulposus cells. Methods Human nucleus pulposus cells were cultured by monolayer and usage of the toluidine blue, safranin O and immunohistochemistry of collagen Ⅱ were identified. And then co-cultured with 10ng/ml and 50ng/ml human recombinant IL-1β for 48 hours, the expression of MMP-1,2, 13 were detected by RT-PCR, MMP-9 were detected by quantitative PCR. Results Recombinant human IL-1β of long and 50 ng/ml could promote matrix metalloproteinases-1,2, 9 and 13 expressions in human nucleus pulposus cells （P〈0.05）. Matrix metallo-proteinases-9,13 expressions were increased with increase of IL- 1β concentration （P〈0.05）. Conclusions IL-1β would promote matrix metalloproteinases-1,2,9 and 13 expressions of human intervertebral disc nucleus pulposus cells to accelerate to decomposition of intervertebral disc matrix. And it also may pass transmission of mentioned about cellular factors.
出处
《岭南现代临床外科》
2009年第1期38-41,共4页
Lingnan Modern Clinics in Surgery
基金
广东省自然科学基金(NO:5001753
5001754
6104608)
广东省科技计划项目(NO:2008B060600059)
关键词
椎间盘
髓核细胞
白介素-1Β
基质金属蛋白酶
Intervertebral disc
Human nucleus pulposus cells
IL- 1β
Matrix metalloproteinase
