摘要
目的:通过观察补肾方对绝经后骨质疏松症动物模型骨胶原PICP、ICTP的影响,探讨其防治骨质疏松症的作用机制。方法:将造模成功后的36只SD大鼠随机分为手术模型组、雌激素组、中药组、假手术模型组。手术模型组给予蒸馏水灌服;中药组给予中药骨康溶液灌服;雌激素组给予尼尔雌醇灌胃(1次/周),其余时间给予蒸馏水10 ml/kg灌胃。经3个月的治疗后,于末次喂药2h后抽取所有老鼠血清,行PICP、ICTP、E2检测。结果:手术模型组与假手术模型组比较,血清E2、PICP含量降低,ICTP含量升高,差异有统计学意义(P<0.05);中药组与手术模型组比较,E2、PICP水平升高,ICTP水平降低,差异有统计学意义(P<0.05),中药组与雌激素组,血清E2、PICP、ICTP比较,差异无统计学意义(P>0.05)。结论:补肾方具有类雌激素样作用,对卵巢切除所致的骨质疏松症有明显的治疗作用,其机制可能在一定程度上有抑制骨吸收,促进骨形成的双重作用。
Objective：To explore the effect of kidney--tonifying formula （Gukang） on serum PICP and ICTP in postmenopausal osteoporosis rats. Methods： Thirty--six female SD rat models built by ovariotomy were distributed randomly into 4 groups： sham--operated group, model group, estrogen group and Chinese herbal medicine group. The sham--operated group and model group were both treated by intragastric administration with distilled water （10ml/kg, once daily）. Chinese herbal medicine group were given intragastric administration with Gukang （10ml/kg, once daily）, while estrogen group were treated by intragastric administration of nilestriol （lmg/kg, once a week）, and distilled water （10ml/kg, once daily） in the rest time during the experiment. After 3 months of treatment, the levels of serum PICP, ICTP and E2 were detected. Resuits .. The content of PICP and E2 were lower while ICTP was higher in model group than in sham--operated group, and the difference was statistically significant （P〈0.05）. Compared with model group, Chinese herbal medicine group had higher content of PICP and E2 and lower content of ICTP （P〈0.05）. And no remarkable differences were observed between the Chinese herbal medicine group and estrogen group in serum PICP, E2 and ICTP （P〉0.05）. Conclusion： Chinese herbal medicine Gukang has similar effect to estrogen on ovariectomy induced osteoporosis, which night be related to the dual functions of inhibiting bone resorption and promoting bone formation.
出处
《中国中医骨伤科杂志》
CAS
2009年第3期13-14,共2页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
