摘要
目的进一步探讨全反式维甲酸(ATRA)对人喉癌的治疗机制。方法取对数生长期人喉癌细胞株Hep-2,以1×105/ml细胞密度接种于96孔培养板,置于CO2培养箱中24h后随机分为观察组和对照组,观察组分别加入终浓度为10-7、10-6、10-5mol/L的ATRA,对照组为空白对照。观察两组细胞增殖抑制率(IR)、细胞凋亡率、Survivin表达水平。结果观察组Hep-2细胞IR及细胞凋亡率均显著高于对照组,Survivin表达显著低于对照组,且呈时间和剂量依赖性。结论ATRA对人喉癌细胞株Hep-2生长有抑制作用,同时可诱导其凋亡,机制可能与Survivin基因表达下调有关。
Objective To investigate the treatment mechanism of all-trans retinoic acid（ATRA） on laryngeal carcinoma. Methods Hep-2 laryngeal carcinoma ceils in logarithmic phase were inoculated into 96 weil-Gibco,and divided into the observed group and the control group, the former was treated by ATRA with the concentration of 10^-7. 10^-6, 10^-5 mmol/L. The inhibition rates （IR） of proliferation were detechted by MTT assay. The apoptosis was analyzed by Hoechst 33258 fluorescence staining. The expression of Survivin was detected through immunoeytochemistry method. Results The IR and apoptosis rates of Hep-2 ceils in observed group were higher than those in the control group , while the expression of Survivin was lower ,all in a close and time dependent manner. Conclusion ATRA can inhibit the growth of Hep-2 laryngeal carcinoma ceils and induce its apoptosis, down-regulation of Survivin gene probably contributes to it.
出处
《山东医药》
CAS
北大核心
2009年第11期30-32,共3页
Shandong Medical Journal
关键词
全反式维甲酸
喉癌细胞
细胞凋亡
生长素
all-trans retinoic acid
laryngeal carcinoma cell
apoptosis
Survivin
