摘要
目的观察全反式维甲酸(atRA)对兔颈动脉内皮损伤后内膜增殖和血管内皮生长因子(VEGF)、血管细胞黏附分子-1(VCAM-1)表达的影响。方法新西兰雄性大白兔36只,随机分为6组:治疗A、B组,对照A、B组,假手术A、B组。治疗组和对照组给予高脂饮食并行颈动脉内膜空气干燥术,假手术组手术暴露颈动脉而不损伤内膜,治疗组给予atRA连续灌胃。分别于术后7、28d处死A、B组动物,取病变血管进行形态学观察。免疫组织化学方法检测病变血管VEGF、VCAM-1表达水平。结果假手术组动脉内膜光滑,无粥样硬化病变及VCAM-1表达。对照组术后7d动脉内膜层出现泡沫细胞,平滑肌细胞增殖,VEGF、VCAM-1表达明显增加,28d时血管内膜明显增厚,有粥样斑块形成。治疗组动脉内膜增生较轻,28d时内膜面积低于对照组(t=4.770,P<0.001),动脉粥样硬化病变轻于对照组(H=4.89,P<0.05),VEGF、VCAM-1表达低于对照组(t=3.280～4.288,P<0.01、0.05)。结论atRA可抑制兔颈动脉内皮损伤后新生内膜增殖,下调VEGF、VCAM-1表达是其作用机制之一。
Objective To observe the effects of all-trans retinoic acid （atRA） on intimal proliferation, the expressions of vascular endothelial growth factor （VEGF） and vascular cell adhesion molecule-1 （VCAM-1） after carotid endothelium injury in rabbits. Methods Methods Thirty-six New Zealand rabbits were randomized to treatment groups （A, B）, control groups （A, B） and sham-operation groups （A, B）. The rabbits in treatment groups and control groups were fed with high cholesterol chow and subjected to carotid endothelial injury by air desiccation. Those in sham-operation groups underwent surgery without endothelium injury, atRA was administered daily to the treatment groups by means of lavage. All rabbits in groups A and B were sacrificed at seven and 28 days after surgery. The carotid specimens were collected for morphology. VEGF and VCAM-1 were detected immunohistochemically. Results In the sham-operation group, the artery intima was smooth, without atherosclerosis and VCAM-1 expression; In the control group, neointimal proliferation started at seven days after surgery, severe intimal hyperplasia and atberosclerosis were found at 28 days, the expressions of VEGF and VCAM-1 were upregulated; In the treatment group, the intimal proliferation at 28 days after surgery was less severe （t=4. 770,P〈0. 001）, and the expressions of VEGF and VCAM-1 were lower than that in the control （t=3. 280-4. 288,P〈0.01,0.05）. Conclusion atRA can inhibit neointimal proliferation after carotid endothelium injury, one of the mechanisms is down regulation of VEGF and VCAM-1 expressions.
出处
《青岛大学医学院学报》
CAS
2009年第2期101-103,106,共4页
Acta Academiae Medicinae Qingdao Universitatis
