摘要
背景:阴茎白膜损伤修复过程中会发生一系列组织形态结构的病理改变,其胶原纤维等结构成分也必然产生相应变化。目的:构建新西兰兔阴茎白膜损伤动物模型,寻找一种简便、快捷、灵敏的观察阴茎白膜损伤愈合过程中胶原类型、分布动态变化的方法。设计、时间及地点:胶原纤维水平的对比观察实验,于2005-06/2007-10在贵阳医学院组织工程实验室完成。材料:新西兰兔15只,均为雄性。方法:在兔阴茎背侧切除白膜5mm×5mm造成缺损制备兔阴茎白膜损伤模型,将切除的白膜原位缝合。分别在损伤后2,6,12周取阴茎白膜,每次5只,以苦味酸天狼猩红染色,应用偏振光显微镜观察。主要观察指标:阴茎白膜损伤愈合过程中胶原纤维面积的动态变化。结果:不同时间点同种纤维含量差异具有显著性意义(P<0.05)。损伤后2周时,Ⅰ型和Ⅲ型胶原纤维共同存在,比例相当,胶原排列杂乱,相互交错。损伤后6周时,术区红色和黄色的Ⅰ型胶原纤维明显增加,Ⅲ型胶原纤维的比例下降,排列欠规律。到损伤后12周,粗大而鲜艳的红黄色Ⅰ型胶原成分占据了绝大部分,细小的绿色Ⅲ型胶原纤维数量急剧减少。结论:苦味酸天狼猩红偏振光法不仅能区分Ⅰ,Ⅲ型胶原的类型,还能清晰地显示Ⅰ,Ⅲ型胶原的形态、分布和比例关系,操作简单,特异性强。
BACKGROUND： Pathological changes of form and structure are induced in some tissues during repairing albuginea penis trauma, while structure and component of collagen fiber will be correspondingly changed necessarily.
OBJECTIVE： To find a convenient, shortcut, and sensitive method to observe the dynamic change of type and distribution of collagen fiber during aibuginea penis healing.
DESIGN, TIME AND SETTING： A contrast study was performed at the Laboratory of Tissue Engineering, Guiyang Medical College between June 2005 and October 2007.
MATERIALS： Fifteen male New Zealand rabbits were used in this study.
METHODS： All rabbits underwent an excision of albuginea sizing 5 mm ×5 mm at dorsal penis to establish models of albuginea penis trauma, and then the excision was sutured in situ. Thereafter, atbuginea penis was obtained from five rabbits once after 2, 6, and 12 weeks. The samples were treated with picric-sirius red staining and observed under polarizing microscope.
MAIN OUTCOME MEASURES： Dynamic changes of area of cotlagen fiber during atbuginea penis heating. RESULTS： There were significant differences in content of the same collagen fiber at different time points （P 〈 0.05）. At 2 weeks post-trauma, both type Ⅰ and type Ⅲ collagen fibers were observed, and the ratio was equal, but the arrangement was confused and disorder. At 6 weeks post-trauma, red and yellow type l collagen fiber was apparently increased, but type Ⅲ collagen fiber was decreased, and the arrangement was still disorder. At 12 weeks post-trauma, thick, bright red and yellow type Ⅰ collagen fiber was rapidly increased, but thin and green type Ⅲ collagen fiber was rapidly decreased.
CONCLUSION： Picric-sirius red polarized light method can not only differentiate types of collagen fiber, but also clearly display the distribution, form and proportion of type Ⅰand type Ⅲ collagen fiber, characterizing by simple, convenient, specific, and highly sensitive.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2009年第11期2187-2190,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
